SDS-Page
Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) Recombinant Protein | SIT1 recombinant protein
Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1); Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1); SIT1 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPKPQASGPEPEL YASVCAQTRR ARASFPDQAY ANSQP
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPKPQASGPEPEL YASVCAQTRR ARASFPDQAY ANSQP
Sequence Length
178
Applicable Applications for SIT1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Ser2~Pro175 (Accession # Q5M869) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Cell membrane; Single-pass type III membrane protein
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.4
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22.8kDa
NCBI Official Full Name
signaling threshold-regulating transmembrane adapter 1
NCBI Official Synonym Full Names
signaling threshold regulating transmembrane adaptor 1
NCBI Official Symbol
Sit1
NCBI Protein Information
signaling threshold-regulating transmembrane adapter 1; suppression inducing transmembrane adaptor 1; suppression-inducing transmembrane adapter 1; SHP2-interacting transmembrane adapter protein
UniProt Protein Name
Signaling threshold-regulating transmembrane adapter 1
Protein Family
UniProt Gene Name
Sit1
UniProt Synonym Gene Names
Sit
UniProt Entry Name
SIT1_RAT
Uniprot Description
SIT: a low molecular weight transmembrane adaptor protein. A disulfide-linked homodimer. Expressed in lymphocytes. Following phosphorylation by Src and possibly Tec kinases, recruits the SH2 domain-containing tyrosine phosphatase SHP2 via an immunoreceptor tyrosine-based inhibition motif (ITIM).
Protein type: Adaptor/scaffold; Membrane protein, integral
Cellular Component: integral to membrane; plasma membrane
Molecular Function: kinase binding
Biological Process: immune system process; regulation of T cell activation
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Product Notes
The SIT1 sit1 (Catalog #AAA2018337) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SIT1 sit1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SRENNCTTA DLAWGIPSIT QAWGLWALFG VVTMLLLISL AALLSQWTRG RRRTQEEQGP PSGRSVEEVP LYGNLHYLQT GRLSEESRSE EQDPSSGGLA RGAEEATCYT SLQLRPAQGR IPSSGTPIKY CEVVLDSEPK PQASGPEPEL YASVCAQTRR ARASFPDQAY ANSQP. It is sometimes possible for the material contained within the vial of "Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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