SIRT6 recombinant protein

Recombinant Human SIRT6 protein

Cat. Num. AAA1561118

• Gene Names: SIRT6; SIR2L6
• Purity: >90% as determined by SDS-PAGE
• Synonyms: SIRT6; Recombinant Human SIRT6 protein; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; SIRT6 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human SIRT6 (Met1-Ser355) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for SIRT6 recombinant protein

Sirtuin 6 (SIRT6) is a nicotinamide adenine dinucleotide(+) (NAD(+)) dependent enzyme and stress response protein. SIRT6 has several different functions in multiple different molecular pathways related to DNA repair, glycolysis, gluconeogenesis, tumorigenesis, neurodegeneration, cardiac hypertrophic responses, and more. SIRT6 is a main regulator of metabolism and lifespan and its importance has been implicated in the prevention against aging-related diseases. SIRT6 depletion enhanced chemosensitivity of human hepatoma cells by downregulating MDR1 expression through suppressing C/EBPbeta. SIRT6 may serve as a novel target to enhance chemosensitivity in HCC cells.

Product Categories/Family for SIRT6 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 38258612
• NCBI GeneID: 51548
• UniProt Accession #: Q8N6T7; O75291; Q6IAF5; Q6PK99; Q8NCD2; Q9BSI5; Q9BWP3; Q9NRC7; Q9UQD1; B2RCD0
• Molecular Weight: 355
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-6
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: SIRT6
• NCBI Official Synonym Symbols: SIR2L6
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6; sirtuin type 6; SIR2-like protein 6; sir2-related protein type 6; regulatory protein SIR2 homolog 6; NAD-dependent deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT6
• UniProt Synonym Gene Names: SIR2L6
• UniProt Entry Name: SIR6_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; EC 2.4.2.31; EC 3.5.1.-; Transferase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear telomeric heterochromatin; nucleolus; nucleus

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; histone deacetylation