Sirtuin 4 (SIRT4) Recombinant Protein | SIRT4 recombinant protein

Recombinant Sirtuin 4 (SIRT4)

Cat. Num. AAA2011882

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Sirtuin 4 (SIRT4); Recombinant Sirtuin 4 (SIRT4); SIRT4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- A GISTESGIPD YRSEKVGLYA RTDRRPIQHI DFIRSAPVRQ RYWARNFVGW PQFSSHQPNP AHWALSNWEK LGKLHWLVTQ NVDALHSKAG NQRLTELHGC MHRVLCLSCG EQTARRVLQD RFQALNPSWS AEAQGVAPDG DVFLTEEQVR SFRVPCCDRC GGPLKPDVVF FGDTVNPDKV DFVHQRVKEA DSLLV
• Sequence Length: 311

• Applicable Applications for SIRT4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ala60~Val255 (Accession # G3V641) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 157821297
• NCBI GeneID: 304539
• NCBI Accession #: NP_001100617.1
• NCBI GenBank Nucleotide #: NM_001107147.1
• UniProt Accession #: G3V641
• Molecular Weight: 23.8kDa
• NCBI Official Full Name: NAD-dependent ADP-ribosyltransferase sirtuin-4
• NCBI Official Synonym Full Names: sirtuin 4
• NCBI Official Symbol: Sirt4
• NCBI Protein Information: NAD-dependent ADP-ribosyltransferase sirtuin-4
• UniProt Protein Name: Protein Sirt4
• Protein Family: NAD-dependent protein deacylase
• UniProt Gene Name: Sirt4
• UniProt Synonym Gene Names: Sirt4_predicted
• UniProt Entry Name: G3V641_RAT

Uniprot Description

Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD+ levels. Down-regulates insulin secretion.