SIRT4 / NAD-Dependent Protein Lipoamidase Sirtuin-4 Recombinant Protein | SIRT4 recombinant protein

Human SIRT4 / NAD-Dependent Protein Lipoamidase Sirtuin-4, Mitochondrial Recombinant Protein

• Gene Names: SIRT4; SIR2L4
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: SIRT4 / NAD-Dependent Protein Lipoamidase Sirtuin-4; Human SIRT4 / NAD-Dependent Protein Lipoamidase Sirtuin-4; Mitochondrial Recombinant Protein; NAD-dependent protein lipoamidase sirtuin-4; mitochondrial; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4; SIR2L4; SIRT4 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 335

• Applicable Applications for SIRT4 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with GLUD1, IDE and SLC25A5 (PubMed:16959573, PubMed:17715127). Interacts with DLAT and PDHX (PubMed:25525879).
• Entry Function: Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 119618588
• NCBI GeneID: 23409
• UniProt Accession #: Q9Y6E7
• NCBI Official Full Name: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), isoform CRA_a
• NCBI Official Synonym Full Names: sirtuin 4
• NCBI Official Symbol: SIRT4
• NCBI Official Synonym Symbols: SIR2L4
• NCBI Protein Information: NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-4
• Protein Family: NAD-dependent protein deacylase
• UniProt Gene Name: SIRT4
• UniProt Entry Name: SIR4_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT4: NAD-dependent protein ADP-ribosyl transferase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1. Inhibits GLUD1 enzyme activity. Down- regulates insulin secretion. Has no detectable protein deacetylase activity. Belongs to the sirtuin family. Class II subfamily.

Protein type: Mitochondrial; Tumor suppressor; Deacetylase; EC 2.4.2.-; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 12q

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; zinc ion binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; chromatin silencing; glutamine metabolic process; positive regulation of lipid biosynthetic process; response to DNA damage stimulus; negative regulation of fatty acid oxidation; negative regulation of insulin secretion