Human CD328 ELISA Kit | SIGLEC7 elisa kit

Human CD328 ELISA Kit

Cat. Num. AAA8248402

• Gene Names: SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
• Reactivity: Human
• Synonyms: CD328; Human CD328 ELISA Kit; AIRM1; Sialic acid-binding Ig-like lectin 7; Siglec-7; Adhesion inhibitory receptor molecule 1; AIRM-1; CDw328; D-siglec; QA79 membrane protein; p75; SIGLEC7 elisa kit

• Reactivity: Human
• Specificity: The Human CD328 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD328 proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 145

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGLEC7 elisa kit

Principle of the Assay: The Human CD328 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD328 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human CD328 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD328 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD328 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD328 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Sialic acid-binding Ig-like lectin 7, also known as CD328 (cluster of differentiation 328) or AIRM1, is a protein that in humans is encoded by the SIGLEC7 gene. And this gene is mapped to chromosome 19. AIRM1 is a novel member of the sialoadhesin family characterized by 3 immunoglobulin-like extracellular domains (1 N-terminal V type and 2 C2 type) and a classic immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic portion. The highest amino acid sequence similarity found was with the myeloid-specific CD33 molecule, and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition.

NCBI and Uniprot Product Information

• NCBI GI #: 469469047
• NCBI GeneID: 27036
• NCBI Accession #: NP_001264130.1
• NCBI GenBank Nucleotide #: NM_001277201.1
• UniProt Accession #: Q9Y286; Q9NZQ1; Q9UJ86; Q9UJ87; Q9Y502
• Molecular Weight: 16,740 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 7 isoform 3
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 7
• NCBI Official Symbol: SIGLEC7
• NCBI Official Synonym Symbols: p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
• NCBI Protein Information: sialic acid-binding Ig-like lectin 7
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 7
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC7
• UniProt Synonym Gene Names: AIRM1; Siglec-7; AIRM-1
• UniProt Entry Name: SIGL7_HUMAN

Uniprot Description

SIGLEC7: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: receptor activity

Biological Process: regulation of immune response