Mouse Sonic hedgehog protein ELISA Kit | SHH elisa kit

Mouse Sonic hedgehog protein (SHH) ELISA Kit

Cat. Num. AAA284691

• Gene Names: Shh; Hx; Dsh; Hhg1; Hxl3; ShhNC; M100081; 9530036O11Rik
• Reactivity: Mouse
• Synonyms: Sonic hedgehog protein; Mouse Sonic hedgehog protein (SHH) ELISA Kit; HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog; SHH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse SHH. No significant cross-reactivity or interference between Mouse SHH and analogues was observed.
• Sequence Length: 462

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for SHH elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SHH in samples. An antibody specific for SHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21617861
• NCBI GeneID: 20423
• NCBI Accession #: NP_033196.1
• NCBI GenBank Nucleotide #: NM_009170.3
• UniProt Accession #: Q62226
• Molecular Weight: 47,773 Da
• NCBI Official Full Name: sonic hedgehog protein
• NCBI Official Synonym Full Names: sonic hedgehog
• NCBI Official Symbol: Shh
• NCBI Official Synonym Symbols: Hx; Dsh; Hhg1; Hxl3; ShhNC; M100081; 9530036O11Rik
• NCBI Protein Information: sonic hedgehog protein
• UniProt Protein Name: Sonic hedgehog protein
• Protein Family: Sonic hedgehog protein
• UniProt Gene Name: Shh
• UniProt Synonym Gene Names: ShhNp

Uniprot Description

SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.

Protein type: Cell cycle regulation; Cell development/differentiation; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 5 B1|5 14.39 cM

Cellular Component: axon; cell soma; cell surface; dendrite; endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus; membrane; membrane raft; nucleus; plasma membrane; proteinaceous extracellular matrix; synaptic vesicle; transport vesicle

Molecular Function: calcium ion binding; glycosaminoglycan binding; hydrolase activity; laminin-1 binding; metal ion binding; patched binding; peptidase activity; protein binding; zinc ion binding

Biological Process: anatomical structure development; anatomical structure formation involved in morphogenesis; androgen metabolic process; angiogenesis; animal organ formation; anterior/posterior pattern specification; axon guidance; Bergmann glial cell differentiation; blood coagulation; branching involved in blood vessel morphogenesis; branching involved in ureteric bud morphogenesis; branching morphogenesis of a tube; camera-type eye development; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; cell development; cell fate commitment; cell fate specification; cell proliferation; cell proliferation in external granule layer; cell-cell signaling; central nervous system development; determination of left/right symmetry; developmental growth; digestive tract mesoderm development; digestive tract morphogenesis; dorsal/ventral neural tube patterning; dorsal/ventral pattern formation; ectoderm development; embryonic development; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic foregut morphogenesis; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; embryonic limb morphogenesis; embryonic morphogenesis; embryonic organ development; embryonic skeletal system development; endocytosis; establishment of cell polarity; forebrain development; forebrain regionalization; formation of anatomical boundary; granule cell precursor proliferation; hair follicle development; hair follicle morphogenesis; heart development; heart looping; hindbrain development; hindgut morphogenesis; inner ear development; intein-mediated protein splicing; intermediate filament organization; kidney development; limb bud formation; limb development; lung development; lymphoid progenitor cell differentiation; male genitalia development; male genitalia morphogenesis; mesenchymal cell proliferation; metanephros development; midbrain development; multicellular organism development; myoblast differentiation; myotube differentiation; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of cell migration; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein catabolic process; negative regulation of T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt signaling pathway; negative thymic T cell selection; neural crest cell migration; neural tube formation; neuroblast proliferation; neuron fate commitment; odontogenesis; odontogenesis of dentine-containing teeth; oligodendrocyte development; oligodendrocyte differentiation; osteoblast development; palate development; pancreas development; pattern specification process; polarity specification of anterior/posterior axis; positive regulation of alpha-beta T cell differentiation; positive regulation of cell differentiation; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of granule cell precursor proliferation; positive regulation of hh target transcription factor activity; positive regulation of immature T cell proliferation in the thymus; positive regulation of mesenchymal cell proliferation; positive regulation of neuroblast proliferation; positive regulation of neuron differentiation; positive regulation of neurotrophin production; positive regulation of oligodendrocyte differentiation; positive regulation of photoreceptor cell differentiation; positive regulation of protein import into nucleus; positive regulation of skeletal muscle cell proliferation; positive regulation of skeletal muscle tissue development; positive regulation of smoothened signaling pathway; positive regulation of striated muscle cell differentiation; positive regulation of T cell differentiation in thymus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of Wnt receptor signaling pathway; positive thymic T cell selection; prostate gland development; proteolysis; regulation of cell proliferation; regulation of gene expression; regulation of odontogenesis; regulation of proteolysis; regulation of transcription, DNA-templated; respiratory tube development; response to axon injury; response to ethanol; signal transduction; skin development; smoothened signaling pathway; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; spinal cord dorsal/ventral patterning; spinal cord motor neuron differentiation; stem cell development; striated muscle cell differentiation; striated muscle tissue development; T cell differentiation in thymus; telencephalon regionalization; thalamus development; thymus development; thyroid gland development; tongue development; tongue morphogenesis; vasculature development; vasculogenesis; ventral spinal cord interneuron specification; Wnt receptor signaling pathway through beta-catenin; zona limitans intrathalamica formation