Sheep vitamin D-binding protein (GC) ELISA Kit | GC elisa kit

Sheep vitamin D-binding protein (GC) ELISA Kit

Cat. Num. AAA7214240

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51
• Reactivity: Sheep
• Synonyms: vitamin D-binding protein (GC); Sheep vitamin D-binding protein (GC) ELISA Kit; GC elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This VDBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep VDBP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GC elisa kit

Principle of the assay: VDBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VDBP antibody and an VDBP-HRP conjugate. The assay sample and buffer are incubated together with VDBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VDBP concentration since VDBP from samples and VDBP-HRP conjugate compete for the anti-VDBP antibody binding site. Since the number of sites is limited, as more sites are occupied by VDBP from the sample, fewer sites are left to bind VDBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VDBP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 324021745
• NCBI GeneID: 2638
• NCBI Accession #: NP_001191236.1
• NCBI GenBank Nucleotide #: NM_001204307.1
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 3
• NCBI Official Synonym Full Names: group-specific component (vitamin D binding protein)
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; DBP/GC; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein; VDB; gc-globulin; vitamin D-binding alpha-globulin; epididymis secretory protein Li 51
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Vitamin D-binding protein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP; VDB
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1