Rabbit anti-Human DYRK1A Polyclonal Antibody | anti-DYRK1A antibody

DYRK1A, NT (Dual Specificity Tyrosine-phosphorylation-regulated Kinase 1A, Protein Kinase Minibrain Homolog, MNBH, HP86, Dual Specificity YAK1-related Kinase, hMNB, DYRK, MNB, MNBH) (Biotin)

Cat. Num. AAA6473300

• Gene Names: DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DYRK1A; Polyclonal Antibody; NT (Dual Specificity Tyrosine-phosphorylation-regulated Kinase 1A; Protein Kinase Minibrain Homolog; MNBH; HP86; Dual Specificity YAK1-related Kinase; hMNB; DYRK; MNB; MNBH) (Biotin); anti-DYRK1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DYRK1A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DYRK1A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DYRK1A (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DYRK1A Antibody (N-term) in 293 cell line lysates (35ug/lane). DYRK1A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DYRK1A Antiboty (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DYRK1A Antiboty (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-DYRK1A antibody

Antibodies; Protein Kinases

References

1. Adayev, T., Biochemistry 46(25), 7614-7624 (2007). 2. Chang, H.S., Int. J. Cancer 120(11), 2377-2385 (2007). 3. Alvarez, M., Mol. Biol. Cell 18(4), 1167-1178 (2007). 4. Wissing, J., Mol. Cell Proteomics 6(3), 537-547 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 18765758
• NCBI GeneID: 1859
• NCBI Accession #: NP_001387
• NCBI GenBank Nucleotide #: NM_001396.3
• UniProt Accession #: Q13627; O60769; Q92582; Q92810; Q9UNM5
• Molecular Weight: 66,099 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
• NCBI Official Symbol: DYRK1A
• NCBI Official Synonym Symbols: MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1A; hMNB; MNB/DYRK protein kinase; serine/threonine kinase MNB; mnb protein kinase homolog hp86; protein kinase minibrain homolog; dual specificity YAK1-related kinase; serine/threonine-specific p
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1A
• UniProt Synonym Gene Names: DYRK; MNB; MNBH; MNBH; hMNB
• UniProt Entry Name: DYR1A_HUMAN

NCBI Description

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.

Protein type: Protein kinase, CMGC; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; CMGC group; DYRK family; Dyrk1 subfamily

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: nucleoplasm; nuclear speck; ribonucleoprotein complex; nucleus

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein self-association; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; tau protein binding; ATP binding; protein kinase activity

Biological Process: circadian rhythm; nervous system development; negative regulation of DNA damage response, signal transduction by p53 class mediator; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitotic cell cycle; protein amino acid phosphorylation; regulation of alternative nuclear mRNA splicing, via spliceosome

Disease: Mental Retardation, Autosomal Dominant 7