Sheep Natriuretic peptides B (NPPB) ELISA Kit | NPPB elisa kit

Sheep Natriuretic peptides B (NPPB) ELISA Kit

Cat. Num. AAA7212689

• Gene Names: NPPB; BNP
• Reactivity: Sheep
• Synonyms: Natriuretic peptides B (NPPB); Sheep Natriuretic peptides B (NPPB) ELISA Kit; NPPB elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of NPPB. No significant cross-reactivity or interference between NPPB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPPB and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 mg/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPPB elisa kit

Intended Uses: This NPPB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep NPPB. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: NPPB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-NPPB antibody and an NPPB-HRP conjugate. The assay sample and buffer are incubated together with NPPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPPB concentration since NPPB from samples and NPPB-HRP conjugate compete for the anti-NPPB antibody binding site. Since the number of sites is limited, as more sites are occupied by NPPB from the sample, fewer sites are left to bind NPPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPPB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NPPB elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4505433
• NCBI GeneID: 4879
• NCBI Accession #: NP_002512.1
• NCBI GenBank Nucleotide #: NM_002521.2
• Molecular Weight: 14,726 Da
• NCBI Official Full Name: natriuretic peptides B preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B; natriuretic protein; brain type natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B
• UniProt Protein Name: Natriuretic peptides B
• Protein Family: Natriuretic peptides
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32
• UniProt Entry Name: ANFB_HUMAN

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Jul 2008]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: diuretic hormone activity; hormone activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; negative regulation of cell growth; regulation of vascular permeability; body fluid secretion; regulation of blood vessel size