Typical Testing Data/Standard Curve (for reference only)
Sheep Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit | DMBT1 elisa kit
Sheep Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DMBT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DMBT1 antibody and an DMBT1-HRP conjugate. The assay sample and buffer are incubated together with DMBT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DMBT1 concentration since DMBT1 from samples and DMBT1-HRP conjugate compete for the anti-DMBT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DMBT1 from the sample, fewer sites are left to bind DMBT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DMBT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. The gene DMBT1 was originally isolated based on its deletion in a medulloblastoma cell line. DMBT1 is expressed with transcripts of 6.0, 7.5, and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung, although the 7.5 kb transcript has not been characterized. The DMBT1 protein is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID). Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor supressor gene, but rather play a role in the interaction of tumor cells and the immune system. [provided by RefSeq, Jul 2008]
Uniprot Description
DMBT1: May be considered as a candidate tumor suppressor gene for brain, lung, esophageal, gastric, and colorectal cancers. May play roles in mucosal defense system, cellular immune defense and epithelial differentiation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. May function as a binding protein in saliva for the regulation of taste sensation. Binds to HIV-1 envelope protein and has been shown to both inhibit and facilitate viral transmission. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly- sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell. Defects in DMBT1 are involved in the development of glioma (GLM). Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Homozygous deletions may be the predominant mechanism of DMBT1 inactivation playing a role in carcinogenesis. DMBT1 is deleted in medulloblastoma and glioblastoma cell lines; point mutations have also been reported in patients with glioma. A loss or reduction of DMBT1 expression has been seen in esophageal, gastric, lung and colorectal carcinomas as well. Belongs to the DMBT1 family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Tumor suppressor; Oncoprotein; Membrane protein, integral; Secreted
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: proteinaceous extracellular matrix; extracellular space; phagocytic vesicle membrane; zymogen granule membrane; extrinsic to membrane; cytoplasm; extracellular region
Molecular Function: protein binding; calcium-dependent protein binding; scavenger receptor activity; pattern recognition receptor activity
Biological Process: induction of bacterial agglutination; receptor-mediated endocytosis; protein transport; epithelial cell differentiation; viral reproduction; innate immune response; positive regulation of epithelial cell differentiation; pattern recognition receptor signaling pathway; defense response to virus; inner cell mass cell proliferation
Disease: Medulloblastoma; Glioma Susceptibility 1
Research Articles on DMBT1
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Product Notes
The Sheep DMBT1 dmbt1 (Catalog #AAA7228501) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7228501 ELISA Kit recognizes Sheep DMBT1. It is sometimes possible for the material contained within the vial of "Deleted in malignant brain tumors 1 protein (DMBT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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