Sheep Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) DIY ELISA Kit | TIMP1 diy elisa kit

Sheep Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit DIY Materials

Cat. Num. AAA2088654

• Gene Names: TIMP1; EPA; EPO; HCI; CLGI; TIMP; TIMP-1
• Reactivity: Sheep
• Synonyms: Tissue Inhibitors Of Metalloproteinase 1 (TIMP1); Sheep Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit DIY Materials; EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor; TIMP1 diy elisa kit

• Reactivity: Sheep
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1). No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) and analogues was observed.
• Sequence Length: 40

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.122ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for TIMP1 diy elisa kit

Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 994731
• NCBI GeneID: 7076
• Molecular Weight: 23,171 Da
• NCBI Official Full Name: tissue inhibitor of metalloproteinase 1, partial
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: TIMP1
• NCBI Official Synonym Symbols: EPA; EPO; HCI; CLGI; TIMP; TIMP-1
• NCBI Protein Information: metalloproteinase inhibitor 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: cytokine activity; metalloendopeptidase inhibitor activity; protease binding; protein binding

Biological Process: extracellular matrix disassembly; negative regulation of catalytic activity; negative regulation of membrane protein ectodomain proteolysis; platelet degranulation; positive regulation of cell proliferation; response to cytokine stimulus; response to hormone stimulus