Sheep Hemoglobin Beta (HBb) DIY ELISA Kit | HBb diy elisa kit

Sheep Hemoglobin Beta (HBb) ELISA Kit DIY Materials

Cat. Num. AAA2089331

• Gene Names: HBB; CD113t-C; beta-globin
• Reactivity: Sheep
• Synonyms: Hemoglobin Beta (HBb); Sheep Hemoglobin Beta (HBb) ELISA Kit DIY Materials; HB-B; HBD; CD113t-C; Beta Globin; Spinorphin; HBb diy elisa kit

• Reactivity: Sheep
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Hemoglobin Beta (HBb). No significant cross-reactivity or interference between Hemoglobin Beta (HBb) and analogues was observed.
• Sequence Length: 30

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma and erythrocyte lysates.
• Detection Range: 6.17-500ug/mL
• Sensitivity: 2.94ug/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for HBb diy elisa kit

Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 410178713
• NCBI GeneID: 3043
• Molecular Weight: 15,998 Da
• NCBI Official Full Name: hemoglobin beta, partial
• NCBI Official Synonym Full Names: hemoglobin subunit beta
• NCBI Official Symbol: HBB
• NCBI Official Synonym Symbols: CD113t-C; beta-globin
• NCBI Protein Information: hemoglobin subunit beta
• UniProt Protein Name: Hemoglobin subunit beta
• Protein Family: Hemoglobin
• UniProt Gene Name: HBB

NCBI Description

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]

Uniprot Description

HBB: Involved in oxygen transport from the lung to the various peripheral tissues. Defects in HBB may be a cause of Heinz body anemias (HEIBAN). This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency. Defects in HBB are the cause of beta-thalassemia (B-THAL). A form of thalassemia. Thalassemias are common monogenic diseases occurring mostly in Mediterranean and Southeast Asian populations. The hallmark of beta-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. Absence of beta chain causes beta(0)-thalassemia, while reduced amounts of detectable beta globin causes beta(+)-thalassemia. In the severe forms of beta-thalassemia, the excess alpha globin chains accumulate in the developing erythroid precursors in the marrow. Their deposition leads to a vast increase in erythroid apoptosis that in turn causes ineffective erythropoiesis and severe microcytic hypochromic anemia. Clinically, beta-thalassemia is divided into thalassemia major which is transfusion dependent, thalassemia intermedia (of intermediate severity), and thalassemia minor that is asymptomatic. Defects in HBB are the cause of sickle cell anemia (SKCA); also known as sickle cell disease. Sickle cell anemia is characterized by abnormally shaped red cells resulting in chronic anemia and periodic episodes of pain, serious infections and damage to vital organs. Normal red blood cells are round and flexible and flow easily through blood vessels, but in sickle cell anemia, the abnormal hemoglobin (called Hb S) causes red blood cells to become stiff. They are C-shaped and resembles a sickle. These stiffer red blood cells can led to microvascular occlusion thus cutting off the blood supply to nearby tissues. Defects in HBB are the cause of beta-thalassemia dominant inclusion body type (B-THALIB). An autosomal dominant form of beta thalassemia characterized by moderate anemia, lifelong jaundice, cholelithiasis and splenomegaly, marked morphologic changes in the red cells, erythroid hyperplasia of the bone marrow with increased numbers of multinucleate red cell precursors, and the presence of large inclusion bodies in the normoblasts, both in the marrow and in the peripheral blood after splenectomy. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytosol; extracellular region; hemoglobin complex

Molecular Function: haptoglobin binding; hemoglobin binding; oxygen binding; peroxidase activity; protein binding

Biological Process: bicarbonate transport; blood coagulation; hydrogen peroxide catabolic process; oxygen transport; protein heterooligomerization; receptor-mediated endocytosis; response to hydrogen peroxide

Disease: Alpha-thalassemia; Beta-thalassemia; Beta-thalassemia, Dominant Inclusion Body Type; Fetal Hemoglobin Quantitative Trait Locus 1; Heinz Body Anemias; Malaria, Susceptibility To; Sickle Cell Anemia