Src Homology 2 Domain Containing Adapter Protein B (SHB) Recombinant Protein | SHB recombinant protein

Recombinant Src Homology 2 Domain Containing Adapter Protein B (SHB)

Cat. Num. AAA2018284

• Gene Names: Shb; BC028832
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Src Homology 2 Domain Containing Adapter Protein B (SHB); Recombinant Src Homology 2 Domain Containing Adapter Protein B (SHB); SHB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SDL KSKAGKGESA GYMEPYEAQR IMTEFQRQES VRSQHKGIQL YDTPYEPEGQ SVDSDSESTV SLRLRESKLP QDDDRPADEY DQPWEWNRVT IPALAAQFNG NEKRQSSPSP SRDRRRQLRA PGGGFKPIKH GSPEFCGILG ERVDPTIPLE KQIWYHGAIS RSDAENLLRL CKECSYLVRNSQTSKHDYSL SLKSNQGFMH MKLAKTKEKY VLGQNSPPFD SVPEVIHYYT TRKL
• Sequence Length: 503

• Applicable Applications for SHB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser248~Leu484 (Accession # Q6PD21) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 244790061
• NCBI GeneID: 230126
• NCBI Accession #: NP_001028478.1
• NCBI GenBank Nucleotide #: NM_001033306.1
• UniProt Accession #: Q6PD21; Q3ULM3; A2AKW3
• Molecular Weight: 30.9kDa
• NCBI Official Full Name: SH2 domain-containing adapter protein B
• NCBI Official Synonym Full Names: src homology 2 domain-containing transforming protein B
• NCBI Official Symbol: Shb
• NCBI Official Synonym Symbols: BC028832
• NCBI Protein Information: SH2 domain-containing adapter protein B
• UniProt Protein Name: SH2 domain-containing adapter protein B
• Protein Family: SH2 domain-containing adapter protein
• UniProt Gene Name: Shb
• UniProt Entry Name: SHB_MOUSE

Uniprot Description

SHB: Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic- FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: membrane; cytoplasm; plasma membrane

Molecular Function: SH3/SH2 adaptor activity; glycoprotein binding

Biological Process: B cell proliferation; blood vessel development; positive regulation of signal transduction; apoptosis; multicellular organismal development; blood vessel morphogenesis; negative regulation of protein kinase activity; hemopoiesis; angiogenesis; positive regulation of mitotic cell cycle; cell differentiation