Sphingomyelin Synthase 2 Recombinant Protein | SGMS2 recombinant protein

Sphingomyelin Synthase 2 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121773

• Gene Names: SGMS2; SMS2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Sphingomyelin Synthase 2; Sphingomyelin Synthase 2 Protein; Human; Recombinant (mFc Tag); Human SMS2/Sphingomyelin synthase 2 Protein (Fc Tag); SMS2 Protein; sphingomyelin synthase 2; SGMS2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr79

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human SGMS2 (NP_689834.1)(Met1-Thr79) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 22749271
• NCBI GeneID: 166929
• NCBI Accession #: NP_689834.1
• NCBI GenBank Nucleotide #: NM_152621.5
• UniProt Accession #: Q8NHU3; A8K2S9; B2RA61
• Molecular Weight: 42,280 Da
• NCBI Official Full Name: phosphatidylcholine:ceramide cholinephosphotransferase 2
• NCBI Official Synonym Full Names: sphingomyelin synthase 2
• NCBI Official Symbol: SGMS2
• NCBI Official Synonym Symbols: SMS2
• NCBI Protein Information: phosphatidylcholine:ceramide cholinephosphotransferase 2
• UniProt Protein Name: Phosphatidylcholine:ceramide cholinephosphotransferase 2
• UniProt Gene Name: SGMS2
• UniProt Synonym Gene Names: SMS2
• UniProt Entry Name: SMS2_HUMAN

NCBI Description

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008]

Uniprot Description

SMS2: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. Belongs to the sphingomyelin synthase family.

Protein type: Lipid Metabolism - sphingolipid; Transferase; Membrane protein, integral; Membrane protein, multi-pass; EC 2.7.8.27

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Golgi apparatus; integral to plasma membrane; plasma membrane; integral to Golgi membrane

Molecular Function: sphingomyelin synthase activity; kinase activity; ceramide cholinephosphotransferase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; sphingomyelin biosynthetic process; phosphorylation