Surfactant Associated Protein D (SPD) Recombinant Protein | SPD recombinant protein

Recombinant Surfactant Associated Protein D (SPD)

Cat. Num. AAA2009718

• Gene Names: Sftpd; SPD; SP-D
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Surfactant Associated Protein D (SPD); Recombinant Surfactant Associated Protein D (SPD); SPD recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-A EMKTLSQRSI TNTCTLVLCS PTENGLPGRD GRDGREGPRG EKGDPGLPGP MGLSGLPGPR GPVGPKGENG SAGEPGPKGE RGLVGPPGSP GISGPAGKEG PSGKQGNIGP QGKPGPKGEA GPKGEVGAPG MQGSAGAKGP AGPKGERGAP GEQGAPGNAG AAGPAGPAGP QGAPGSRGPP GLKGDRGAPG DRGIKGESGL PDSAALRQQM EALNGKLQRL EAAFSRYKKA ALFPDGQSVG DKIFRAANSE EPFEDAKEMC RQAGGQLASP RSATENAAVQ QLVTAHSKAA FLSMTDVGTE GKFTYPTGEA LVYSNWAPGE PNNNGGAENC VEIFTNGQWN DKACGEQRLV ICEF
• Sequence Length: 374

• Applicable Applications for SPD recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ala20~Phe374 (Accession # P35248) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7949133
• NCBI GeneID: 25350
• NCBI Accession #: NP_037010.1
• NCBI GenBank Nucleotide #: NM_012878.2
• UniProt Accession #: P35248
• Molecular Weight: 37.0kDa
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: Sftpd
• NCBI Official Synonym Symbols: SPD; SP-D
• NCBI Protein Information: pulmonary surfactant-associated protein D; CP4; PSP-D; lung surfactant protein D; pulmonary surfactant protein D; surfactant associated protein D; surfactant, pulmonary-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: Sftpd
• UniProt Synonym Gene Names: Sftp4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_RAT

NCBI Description

binds carbohydrates; may play a role in defense response in the lung [RGD, Feb 2006]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: multivesicular body; proteinaceous extracellular matrix; extracellular space; collagen; rough endoplasmic reticulum; cytoplasm; cytoplasmic vesicle

Molecular Function: monosaccharide binding; identical protein binding; lipopolysaccharide binding; carbohydrate binding

Biological Process: negative regulation of phagocytosis; negative regulation of T cell proliferation; response to hyperoxia; positive regulation of phagocytosis; response to glucocorticoid stimulus; innate immune response; regulation of liquid surface tension; respiratory gaseous exchange; opsonization; surfactant homeostasis; alveolus development; negative regulation of interleukin-2 production; response to corticosteroid stimulus