Human Secreted Frizzled-Related Protein 1 ELISA Kit | SFRP1 elisa kit

Human Secreted Frizzled-Related Protein 1 ELISA Kit

Cat. Num. AAA14040

• Gene Names: SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
• Reactivity: Human
• Synonyms: Secreted Frizzled-Related Protein 1; Human Secreted Frizzled-Related Protein 1 ELISA Kit; SFRP1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human SFRP1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SFRP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SFRP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SFRP1 and SFRP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SFRP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SFRP1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SFRP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SFRP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SFRP1 and SFRP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SFRP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56117838
• NCBI GeneID: 6422
• NCBI Accession #: NP_003003.3
• NCBI GenBank Nucleotide #: NM_003012.4
• UniProt Accession #: Q8N474; O00546; O14779
• Molecular Weight: 35,386 Da
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled-related protein 1
• NCBI Official Symbol: SFRP1
• NCBI Official Synonym Symbols: FRP; FRP1; FrzA; FRP-1; SARP2
• NCBI Protein Information: secreted frizzled-related protein 1; SARP-2; sFRP-1; secreted apoptosis-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 1
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP1
• UniProt Synonym Gene Names: FRP; FRP1; SARP2; FRP-1; sFRP-1; SARP-2
• UniProt Entry Name: SFRP1_HUMAN

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]

Uniprot Description

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; extracellular region; plasma membrane; integral to membrane; intracellular; cytosol

Molecular Function: G-protein coupled receptor activity; heparin binding; Wnt-protein binding; identical protein binding; Wnt receptor activity; protein binding; frizzled binding; cysteine-type endopeptidase activity; drug binding

Biological Process: negative regulation of osteoclast differentiation; negative regulation of Wnt receptor signaling pathway; somatic stem cell maintenance; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of insulin secretion; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of cell proliferation; positive regulation of stress fiber formation; negative regulation of ossification; positive regulation of smoothened signaling pathway; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; negative regulation of cell migration; positive regulation of Wnt receptor signaling pathway; female gonad development; negative regulation of epithelial cell proliferation; negative regulation of B cell differentiation; response to drug; negative regulation of JNK activity; negative regulation of peptidyl-tyrosine phosphorylation; menstrual cycle phase; male gonad development; digestive tract morphogenesis; cellular response to starvation; positive regulation of cell growth; regulation of angiogenesis; osteoblast differentiation; negative regulation of fibroblast proliferation; neural tube closure; negative regulation of bone remodeling; positive regulation of fat cell differentiation; negative regulation of osteoblast proliferation; hemopoietic progenitor cell differentiation; neural crest cell fate commitment; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis