Typical Testing Data/Standard Curve (for reference only)
()
Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit | sFLt-1 elisa kit
Rat Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: sFLTR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sFLTR-1 antibody and an sFLTR-1-HRP conjugate. The assay sample and buffer are incubated together with sFLTR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLTR-1 concentration since sFLTR-1 from samples and sFLTR-1-HRP conjugate compete for the anti-sFLTR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLTR-1 from the sample, fewer sites are left to bind sFLTR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLTR-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.
Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; VEGFR family
Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; integral to membrane; endosome; receptor complex
Molecular Function: transferase activity; identical protein binding; vascular endothelial growth factor receptor activity; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity
Biological Process: cell migration; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; chemotaxis; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; response to hypoxia; blood vessel morphogenesis; embryonic morphogenesis; angiogenesis; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration
Research Articles on sFLt-1
Similar Products
Product Notes
The Rat sFLt-1 flt1 (Catalog #AAA725733) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725733 ELISA Kit recognizes Rat sFLt-1. It is sometimes possible for the material contained within the vial of "Soluble Fms-like tyrosine kinase Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
