Phosphoinositide 3 Kinase, p85 alpha, p110 delta Active Protein | PIK3CD active protein

Phosphoinositide 3 Kinase, p85 alpha, p110 delta, Recombinant, Human (PI3K)

Cat. Num. AAA635734

• Gene Names: PIK3CD; PI3K; p110D; P110DELTA
• Purity: Highly Purified; 95% (SDS-PAGE)
• Synonyms: Phosphoinositide 3 Kinase; p85 alpha; p110 delta; Recombinant; Human (PI3K); PIK3CD active protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2, and 50% glycerol.

• Biological Activity: ~ 3 nmol/mg/min using phosphatidylinositol as the substrate.
• Preparation and Storage: Recombinant Human PI3Kd although stable at 14 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.

Related Product Information for PIK3CD active protein

PI3K is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3K -/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8alpha-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3K. Distinct regions within the p101 regulatory subunit primary stucture are responsible for interaction with PI3K and Gbg. The PI3K binding site is confined to the N-terminus, whereas binding to Gbg is mediated by the C-terminus of p101. The PI3K catalytic subunit carries a N-terminal His6 affinity Tag, whereas the p101 adaptor subunit carries a N-terminal GST Tag. The molecular weight for p110g chain is 126.3kD (without Tag) and the p101 chain 101kD (without Tag).

Product Categories/Family for PIK3CD active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 156564405
• NCBI GeneID: 5293
• NCBI Accession #: NP_005017.3
• NCBI GenBank Nucleotide #: NM_005026.3
• UniProt Accession #: O00329; O15445; Q5SR49; A6NCG0; G1FFP1
• Molecular Weight: p110 chain 120.2kD (without Tag); p85 chain 83.5kD
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• NCBI Official Synonym Full Names: phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta
• NCBI Official Symbol: PIK3CD
• NCBI Official Synonym Symbols: PI3K; p110D; P110DELTA
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3Kdelta; phosphoinositide-3-kinase C; PI3-kinase p110 subunit delta; ptdIns-3-kinase subunit p110-delta; phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CD
• UniProt Synonym Gene Names: PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta
• UniProt Entry Name: PK3CD_HUMAN

NCBI Description

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.[provided by RefSeq, Jul 2010]

Uniprot Description

PIK3CD: Phosphoinositide-3-kinase (PI3K) that phosphorylates PftdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Have a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Have important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity. Belongs to the PI3/PI4-kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; EC 2.7.1.153; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: mast cell granule; plasma membrane; phosphoinositide 3-kinase complex; cytosol

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; ATP binding; phosphoinositide 3-kinase activity

Biological Process: respiratory burst during defense response; epidermal growth factor receptor signaling pathway; neutrophil chemotaxis; adaptive immune response; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell activation; nerve growth factor receptor signaling pathway; mast cell chemotaxis; natural killer cell activation; cytokine production; mast cell degranulation; signal transduction; protein amino acid phosphorylation; T cell receptor signaling pathway; B cell receptor signaling pathway; B cell homeostasis; phosphoinositide phosphorylation; natural killer cell differentiation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; innate immune response; inflammatory response; T cell differentiation

Disease: Immunodeficiency 14