pla2g4a active protein
PLA2G4A, Recombinant, Human (Cytosolic Phospholipase A2, cPLA2, Phospholipase A2 Group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4, CPLA2)
Gene Names
pla2g4a; cPLA2; pla2g4
Purity
Purified~80% by SDS-PAGE under reducing conditions and visualized by colloidial Coomassie Blue stain at 5ug per lane. Endotoxin: 1EU/1ug (LAL)
Synonyms
PLA2G4A; Recombinant; Human (Cytosolic Phospholipase A2; cPLA2; Phospholipase A2 Group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase; PLA2G4; CPLA2); pla2g4a active protein
Host
Sf21cells
Purity/Purification
Purified
~80% by SDS-PAGE under reducing conditions and visualized by colloidial Coomassie Blue stain at 5ug per lane. Endotoxin: 1EU/1ug (LAL)
~80% by SDS-PAGE under reducing conditions and visualized by colloidial Coomassie Blue stain at 5ug per lane. Endotoxin: 1EU/1ug (LAL)
Form/Format
Supplied as a liquid in 25mM Tris, 400mM sodium chloride, 10mM DTT, 0.02% Brij-35, pH 7.5
Biological Activity
Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine. The specific activity is >30pmol/min/ug.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for pla2g4a active protein
Cytosolic Phospholipase A2a (PLA2G4A) belongs to the group IV phospholipase A2 family (1). These enzymes hydrolyze the ester bond at the second position (sn-2) of membrane glycerophospholipids. The group IV phospholipase A2 (PLA2G4) family is comprised of six intracellular enzymes (2). PLA2G4A has a preference for arachidonic acid in the sn-2 position of phospholipids indicating its involvement in the metabolism of eicosanoids. Its active site is characterized by a serine and aspartic acid dyad. The overall structural feature displays two domains, a lipid binding C2 domain and a catalytic a/b hydrolase domain. PLA2G4A translocation to the membrane and activation is facilitated by a calcium-ion dependent conformational change in its C2 domain. In addition, phosphorylation of serine residues in PLA2G4A by protein kinases may be another regulatory mechanism for promoting the release of arachidonic acid. The knockout mouse studies of this enzyme indicate that the enzyme may play a major role in inflammatory diseases. Genetic ablation or reduction of PLA2G4A protected human amyloid precursor protein (APP) related pathogenesis from a mouse model (3). Mouse model studies of brain and lung cancer indicates that PLA2G4A may play a key regulatory role in angiogenesis of these tumors (4).
Recombinant corresponding to aa1-749 from Human PLA2G4A fused with 6-His-tag at C-terminal expressed in Sf21 cells (NP_077734).
Recombinant corresponding to aa1-749 from Human PLA2G4A fused with 6-His-tag at C-terminal expressed in Sf21 cells (NP_077734).
Product Categories/Family for pla2g4a active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~86kD
NCBI Official Full Name
pla2g4a protein
NCBI Official Synonym Full Names
phospholipase A2, group IVA (cytosolic, calcium-dependent)
NCBI Official Symbol
pla2g4a
NCBI Official Synonym Symbols
cPLA2; pla2g4
NCBI Protein Information
cytosolic phospholipase A2; Phospholipase A2 group IVA
UniProt Protein Name
Cytosolic phospholipase A2
Protein Family
UniProt Gene Name
pla2g4a
UniProt Synonym Gene Names
cpla2; pla2g4; cPLA2
UniProt Entry Name
PA24A_XENTR
Uniprot Description
Function: Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response
By similarity.
Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Enzyme regulation: Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+
By similarity.
Subcellular location: Cytoplasm
By similarity. Cytoplasmic vesicle
By similarity. Note: Translocates to membrane vesicles in a calcium-dependent fashion
By similarity.
Domain: The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+
By similarity.
Sequence similarities: Contains 1 C2 domain.Contains 1 PLA2c domain.
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Product Notes
The pla2g4a pla2g4a (Catalog #AAA637489) is an Active Protein produced from Sf21cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PLA2G4A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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