CREB active protein

CREB binding protein (mouse), (recombinant) (His-tag)

Cat. Num. AAA566081

• Gene Names: Crebbp; CBP; KAT3A; AW558298; CBP/p300; p300/CBP
• Purity: >=95% (SDS-PAGE)
• Synonyms: CREB; CREB binding protein (mouse); (recombinant) (His-tag); CREB active protein

• Host: SF21 Insect cells
• Purity/Purification: >=95% (SDS-PAGE)
• Form/Format: Liquid. In 50mM TRIS-HCl, pH7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol
• Concentration: 0.5mg/ml (varies by lot)

• Source Note: Fused at the C-terminus to a His-tag. Produced in a baculovirus expression system
• Specific Activity: ~50-100ng are required for standard HAT assay
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C. Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen; Shipping: Shipped on Dry Ice

Product Categories/Family for CREB active protein

Cellular Analysis; DNA Regulation & Transcription; Stem Cells; Epigenetics; Histone Posttranslational Modifications

NCBI and Uniprot Product Information

• NCBI GI #: 341940549
• NCBI GeneID: 12914
• UniProt Accession #: P45481; E9QPH4
• Molecular Weight: 265,494 Da
• NCBI Official Full Name: CREB-binding protein
• NCBI Official Synonym Full Names: CREB binding protein
• NCBI Official Symbol: Crebbp
• NCBI Official Synonym Symbols: CBP; KAT3A; AW558298; CBP/p300; p300/CBP
• NCBI Protein Information: CREB-binding protein
• UniProt Protein Name: CREB-binding protein
• Protein Family: CREB-binding protein
• UniProt Gene Name: Crebbp
• UniProt Synonym Gene Names: Cbp

Uniprot Description

CBP: a protein acetyltransferase that can transcriptionally activate histones. Acetylates the NCOA3 coactivator. Binds specifically to phosphorylated CREB1 and enhances its transcriptional activity toward cAMP-responsive genes. Methylation of the KIX domain by CARM1 blocks association with CREB, blocking CREB signaling, and activating the apoptotic response. Found in a complex containing NCOA2, NCOA3, IKKA, IKKB, and IKBKG. Probably part of a complex with HIF1A and EP300. Interacts with the C-terminal region of CITED4. The TAZ-type 1 domain interacts with HIF1A. Interacts with MAF, SRCAP, CARM1, ELF3, MLLT7/FOXO4, N4BP2, NCOA1, NCOA3, NCOA6, PCAF, PELP1, PML, SMAD1, SMAD2, SMAD3, SPIB and TRERF1. Interacts with HTLV-1 Tax, p30II, and HIV-1 Tat. Interacts with KLF1; the interaction results in acetylation of KLF1 and enhancement of its transcriptional activity. Interacts with ZCCHC12. Interacts with DAXX; the interaction is dependent on CBP sumoylation and results in suppression of the transcriptional activiy via recruitment of HDAC2 to DAAX. Interacts with MTDH. Interacts with NFATC4. Interacts with MAFG; the interaction acetylates MAFG in the basic region and stimulates NFE2 transcriptional activity through increasing its DNA-binding activity. Interacts with IRF2; the interaction acetylates IRF2 and regulates its activity on the H4 promoter. Interacts via its N-terminus with the C-terminus of SS18L1. Interacts with MECOM. Chromosomal aberrations involving CBP may be a cause of acute myeloid leukemias. Known translocation partners include MYST3, MLL, and MYST4. MYST3-CBP fusion proteins may induce leukemia by inhibiting RUNX1-mediated transcription. Defects in CBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1), an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.

Protein type: Acetyltransferase; DNA-binding; EC 2.3.1.48; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 16 A1|16 2.4 cM

Cellular Component: cytoplasm; histone acetyltransferase complex; nuclear body; nuclear chromatin; nucleoplasm; nucleus; outer kinetochore of condensed chromosome; PML body; protein complex; RNA polymerase II transcription factor complex; transcription factor complex

Molecular Function: acetyltransferase activity; activating transcription factor binding; cAMP response element binding protein binding; chromatin binding; damaged DNA binding; DNA binding; DNA binding transcription factor activity; histone acetyltransferase activity; metal ion binding; MRF binding; p53 binding; peroxisome proliferator activated receptor binding; protein binding; protein domain specific binding; protein heterodimerization activity; SMAD binding; transcription coactivator activity; transcription cofactor activity; transcription factor binding; transferase activity; transferase activity, transferring acyl groups; zinc ion binding

Biological Process: cell proliferation; cellular response to UV; germ-line stem cell maintenance; histone acetylation; N-terminal peptidyl-lysine acetylation; negative regulation of interferon-beta biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral process; positive regulation of CREB transcription factor activity; positive regulation of G1/S transition of mitotic cell cycle; positive regulation of gene expression; positive regulation of NIK/NF-kappaB signaling; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein amino acid acetylation; protein destabilization; regulation of transcription, DNA-templated; rhythmic process; transcription, DNA-dependent