Human SET domain and mariner transposase fusion gene ELISA Kit | SETMAR elisa kit

Human Histone-lysine N-methyltransferase SETMAR, SETMAR ELISA Kit

Cat. Num. AAA9317447

• Gene Names: SETMAR; Mar1; HsMar1; METNASE
• Reactivity: Human
• Synonyms: SET domain and mariner transposase fusion gene; Human Histone-lysine N-methyltransferase SETMAR; SETMAR ELISA Kit; Human Histone-lysine N-methyltransferase SETMAR (SETMAR) ELISA kit; METNASE; SET domain and mariner transposase fusion; SETMAR elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human SETMAR and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SETMAR concentrations in Human serum, plasma and other body fluids. Using Purified Human SETMAR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SETMAR and SETMAR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SETMAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SETMAR elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SETMAR concentrations in Human serum, plasma and other body fluids. Using Purified Human SETMAR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SETMAR and SETMAR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SETMAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 344030209
• NCBI GeneID: 6419
• NCBI Accession #: NP_001230652.1
• NCBI GenBank Nucleotide #: NM_001243723.1
• UniProt Accession #: Q53H47; Q13579; Q1G668; Q96F41; B4DY74; E7EN68
• Molecular Weight: 78,034 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETMAR isoform 2
• NCBI Official Synonym Full Names: SET domain and mariner transposase fusion gene
• NCBI Official Symbol: SETMAR
• NCBI Official Synonym Symbols: Mar1; HsMar1; METNASE
• NCBI Protein Information: histone-lysine N-methyltransferase SETMAR; SET domain and mariner transposase fusion gene-containing protein
• UniProt Protein Name: Histone-lysine N-methyltransferase SETMAR
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETMAR
• UniProt Synonym Gene Names: HsMar1; Metnase
• UniProt Entry Name: SETMR_HUMAN

NCBI Description

This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

SETMAR: Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 3p26.1

Cellular Component: condensed chromosome; nucleus

Molecular Function: protein binding; protein homodimerization activity; structure-specific DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); endonuclease activity; double-stranded DNA binding; single-stranded DNA binding; transposase activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: cell proliferation; DNA integration; histone H3-K4 methylation; DNA double-strand break processing; histone H3-K36 methylation; transposition, DNA-mediated; DNA catabolic process, endonucleolytic; double-strand break repair via nonhomologous end joining; replication fork processing