Human Neuroserpin ELISA Kit | SERPINI1 elisa kit

Neuroserpin (Human) OmniKine ELISA Kit

Cat. Num. AAA9502108

• Gene Names: SERPINI1; PI12; neuroserpin
• Reactivity: Human
• Synonyms: Neuroserpin; Neuroserpin (Human) OmniKine ELISA Kit; Serpin Family I Member; Serine (Or Cysteine) Proteinase Inhibitor; Clade I (Neuroserpin); Member 1; Serpin Peptidase Inhibitor; Peptidase Inhibitor 12; Serpin I1; PI-12; PI12; Serpin Peptidase Inhibitor Clade I Member 1; SERPINI1 elisa kit

• Reactivity: Human
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 410

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for SERPINI1 elisa kit

Principle of the Assay: The OmniKine™ Human Neuroserpin ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Human Neuroserpin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Neuroserpin while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Human Neuroserpin is a member of the serpin superfamily of serine proteinase inhibitors and is encoded by the SERPINI1 gene. Found to be secreted by axons in the brain it is thought to play a role in regulation of axonal growth and development of synaptic plasticity.

NCBI and Uniprot Product Information

• NCBI GI #: 170295807
• NCBI GeneID: 5274
• NCBI Accession #: NP_001116224.1
• NCBI GenBank Nucleotide #: NM_001122752.1
• UniProt Accession #: Q99574; Q6AHZ4; A8K217; D3DNP1
• Molecular Weight: 46,427 Da
• NCBI Official Full Name: neuroserpin
• NCBI Official Synonym Full Names: serpin family I member 1
• NCBI Official Symbol: SERPINI1
• NCBI Official Synonym Symbols: PI12; neuroserpin
• NCBI Protein Information: neuroserpin
• UniProt Protein Name: Neuroserpin
• Protein Family: Neuroserpin
• UniProt Gene Name: SERPINI1
• UniProt Synonym Gene Names: PI12; PI-12

NCBI Description

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q26.1

Cellular Component: cell soma; extracellular space; perikaryon

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: central nervous system development; peripheral nervous system development; regulation of cell adhesion

Disease: Encephalopathy, Familial, With Neuroserpin Inclusion Bodies