Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Glia-derived nexin (SERPINE2) Standard Curve.)
Human Glia-Derived Nexin (SERPINE2) ELISA Kit | GDN elisa kit
Human Glia-Derived Nexin (SERPINE2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Glia-derived nexin (SERPINE2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Glia-derived nexin (SERPINE2) Standard Curve.)
Background: Protease nexin I is a 44-kD thrombin and urokinase inhibitor released by human foreskin fibroblasts. PN-I shares several features with antithrombin III, an abundant plasma thrombin inhibitor. Both PN-I and AT-III have high affinities for heparin, and heparin accelerates their rate of thrombin inhibition. In addition, the published sequence of 28 amino acids at the N-terminus of PN-I is identical to the N-terminal amino acid sequence of a glial-derived neurite promoting factor. Two forms, designated alpha and beta, were identified. These differed only by the insertion of 3 nucleotides into the coding sequence, and apparently arose by utilization of an alternative splice acceptor site in the PN1 gene. In the resulting proteins, alpha-PN-I contains an arginine residue at position 310, whereas beta-PN-I contains threonine-lysine residues.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
SERPINE2: Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q36.1
Cellular Component: cell soma; cytosol; extracellular matrix; extracellular region; extracellular space; extrinsic to external side of plasma membrane; neuromuscular junction
Molecular Function: glycosaminoglycan binding; heparin binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity
Biological Process: blood coagulation; cerebellar granular layer morphogenesis; detection of mechanical stimulus involved in sensory perception; embryo implantation; mating plug formation; negative regulation of blood coagulation; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of proteolysis; negative regulation of smoothened signaling pathway; positive regulation of astrocyte differentiation; regulation of cell migration; regulation of synaptic transmission, glutamatergic; regulation of timing of cell differentiation; response to radiation; secretory granule organization and biogenesis
Research Articles on GDN
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Product Notes
The Human GDN serpine2 (Catalog #AAA9714358) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714358 ELISA Kit recognizes Human GDN. It is sometimes possible for the material contained within the vial of "Glia-Derived Nexin (SERPINE2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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