Mammary Serine Protease Inhibitor Recombinant Protein | Maspin recombinant protein

Recombinant Mammary Serine Protease Inhibitor (Maspin)

Cat. Num. AAA2029225

• Gene Names: SERPINB5; PI5; maspin
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Mammary Serine Protease Inhibitor; Recombinant Mammary Serine Protease Inhibitor (Maspin); Maspin recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFSESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRHNKTRNIIFFG KFCSP
• Sequence Length: 375

• Applicable Applications for Maspin recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 45.8kDa
• Accurate Molecular Mass (KD): 46kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Pro375
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 453369
• NCBI GeneID: 5268
• Molecular Weight: 25,763 Da
• NCBI Official Full Name: maspin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• NCBI Official Symbol: SERPINB5
• NCBI Official Synonym Symbols: PI5; maspin
• NCBI Protein Information: serpin B5
• UniProt Protein Name: Serpin B5
• UniProt Gene Name: SERPINB5
• UniProt Synonym Gene Names: PI5; PI-5
• UniProt Entry Name: SPB5_HUMAN

Uniprot Description

maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: extracellular space; cytoplasm

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding

Biological Process: extracellular matrix organization and biogenesis; morphogenesis of an epithelium; cell motility; regulation of epithelial cell proliferation