Camel Semenogelin I ELISA Kit | SEMG1 elisa kit

Camel Semenogelin I ELISA Kit

Cat. Num. AAA47619

• Gene Names: SEMG1; SGI; SEMG; CT103; dJ172H20.2
• Reactivity: Camel
• Synonyms: Semenogelin I; Camel Semenogelin I ELISA Kit; SEMG1 elisa kit

• Reactivity: Camel
• Specificity: No significant cross-reactivity or interference between Camel SEMG1 and analogues was observed.
• Sequence Length: 462

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SEMG1 elisa kit

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SEMG1 concentrations in Camel serum, plasma and other body fluids. Using Purified Camel SEMG1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SEMG1 and SEMG1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SEMG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 338019
• NCBI GeneID: 6406
• UniProt Accession #: P04279; Q53ZV0; Q53ZV1; Q53ZV2; Q6X4I9; Q6Y809; Q6Y822; Q6Y823; Q86U64; Q96QM3
• Molecular Weight: 52,131 Da
• NCBI Official Full Name: semenogelin
• NCBI Official Synonym Full Names: semenogelin I
• NCBI Official Symbol: SEMG1
• NCBI Official Synonym Symbols: SGI; SEMG; CT103; dJ172H20.2
• NCBI Protein Information: semenogelin-1; cancer/testis antigen 103; semen coagulating protein
• UniProt Protein Name: Semenogelin-1
• Protein Family: Semenogelin
• UniProt Gene Name: SEMG1
• UniProt Synonym Gene Names: SEMG; SGI
• UniProt Entry Name: SEMG1_HUMAN

NCBI Description

The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. [provided by RefSeq, Jul 2009]

Uniprot Description

SEMG1: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Belongs to the semenogelin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; extracellular region; nucleus; secretory granule

Molecular Function: protein binding; structural molecule activity

Biological Process: insemination