SECTM1 / K12 recombinant protein

Recombinant Human SECTM1 / K12 Protein (His tag)

Cat. Num. AAA2545530

• Gene Names: SECTM1; K12
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: SECTM1 / K12; Recombinant Human SECTM1 / K12 Protein (His tag); K12; SECTM1 / K12 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 248

• Application Notes: The recombinant human SECTM1 consists of 128 amino acids and has a predicted molecular mass of 14 kDa. As a result of glycosylation, the apparent molecular mass of rhSECTM1 is approximately 23 kDa in SDS-PAGE under reducing conditions
• Predicted N Terminal: Gln 29
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SECTM1 / K12 recombinant protein

Background: Secreted and transmembrane 1 (SECTM1), also known as K12, is a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein of SECTM family. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The human K12 protein has been shown to be primarily expressed in spleen, prostate, testis, small intestine, and in peripheral blood leukocytes. The K12 protein is expressed on the cell surface in such small amounts as to preclude detection. Alternatively, it may be that K12 on the cell surface is rapidly cleaved to generate a soluble K12 protein. Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. May be involved in thymocyte signaling. It had been suggested a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis. In addition, as a putative natural CD7 ligand, SECTM1/K12 may be responsible for the costimulatory role it plays in T cell activation.

Description: A DNA sequence encoding the human SECTM1 (Q8WVN6-1) extracellular domain (Met 1-Gly 145) was fused with a polyhistidine tag at the C-terminus

NCBI and Uniprot Product Information

• NCBI GI #: 4506869
• NCBI GeneID: 6398
• NCBI Accession #: NP_002995.1
• NCBI GenBank Nucleotide #: NM_003004.2
• UniProt Accession #: Q8WVN6; O00466; B2R7H0
• Molecular Weight: 27,039 Da
• NCBI Official Full Name: secreted and transmembrane protein 1
• NCBI Official Synonym Full Names: secreted and transmembrane 1
• NCBI Official Symbol: SECTM1
• NCBI Official Synonym Symbols: K12
• NCBI Protein Information: secreted and transmembrane protein 1
• UniProt Protein Name: Secreted and transmembrane protein 1
• UniProt Gene Name: SECTM1
• UniProt Synonym Gene Names: K12

NCBI Description

This gene encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. [provided by RefSeq, Jul 2008]

Uniprot Description

SECTM1: May be involved in thymocyte signaling. Belongs to the SECTM family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular space; Golgi apparatus; integral to membrane

Molecular Function: signal transducer activity

Biological Process: mesoderm development; positive regulation of I-kappaB kinase/NF-kappaB cascade