Human Secreted And Transmembrane Protein 1 (SECTM1) ELISA Kit | SECTM1 elisa kit

Human Secreted And Transmembrane Protein 1 (SECTM1) ELISA Kit

Cat. Num. AAA2707056

• Gene Names: SECTM1; K12
• Reactivity: Human
• Synonyms: Secreted And Transmembrane Protein 1 (SECTM1); Human Secreted And Transmembrane Protein 1 (SECTM1) ELISA Kit; K12; Type 1a Transmembrane Protein; SECTM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secreted And Transmembrane Protein 1 (SECTM1).; No significant cross-reactivity or interference between Secreted And Transmembrane Protein 1 (SECTM1) and analogues was observed.
• Sequence Length: 248

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Urine and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted And Transmembrane Protein 1 (SECTM1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted And Transmembrane Protein 1 (SECTM1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SECTM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted And Transmembrane Protein 1 (SECTM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted And Transmembrane Protein 1 (SECTM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted And Transmembrane Protein 1 (SECTM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted And Transmembrane Protein 1 (SECTM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506869
• NCBI GeneID: 6398
• NCBI Accession #: NP_002995.1
• NCBI GenBank Nucleotide #: NM_003004.2
• UniProt Accession #: Q8WVN6; O00466; B2R7H0
• Molecular Weight: 27,039 Da
• NCBI Official Full Name: secreted and transmembrane protein 1
• NCBI Official Synonym Full Names: secreted and transmembrane 1
• NCBI Official Symbol: SECTM1
• NCBI Official Synonym Symbols: K12
• NCBI Protein Information: secreted and transmembrane protein 1
• UniProt Protein Name: Secreted and transmembrane protein 1
• Protein Family: Secreted and transmembrane protein
• UniProt Gene Name: SECTM1
• UniProt Synonym Gene Names: K12

NCBI Description

This gene encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. [provided by RefSeq, Jul 2008]

Uniprot Description

SECTM1: May be involved in thymocyte signaling. Belongs to the SECTM family.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular space; Golgi apparatus; integral component of membrane; plasma membrane

Molecular Function: cytokine activity; signal transducer activity

Biological Process: immune response; mesoderm development; positive regulation of I-kappaB kinase/NF-kappaB signaling; signal transduction