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Western Blot (WB)
(Mouse IgG were subjected to SDS PAGE followed by western blot with FNSA-0003 at dilution of 1:1000)
IgG (H+L) Secondary Antibody
HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L)
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Synonyms
IgG (H+L); HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L); IgG (H+L) secondary antibody
Purity/Purification
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Form/Format
Liquid
Applicable Applications for IgG (H+L) secondary antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suggested dilution: 1:1000-1:10000 for western blotting with ECL substrates; 1:1000-1:20000 for ELISA and Western blotting with chromogenic substrates; 1:20-1:50 for IHC
Conjugation
HRP purified from horseradish root
Preparation and Storage
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. -20 degree C for 24 months.
Western Blot (WB)
(Mouse IgG were subjected to SDS PAGE followed by western blot with FNSA-0003 at dilution of 1:1000)
Western Blot (WB)
(Mouse IgG were subjected to SDS PAGE followed by western blot with FNSA-0003 at dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse lung cancer using PCK antibody and FNSA-0003 (at dilution of 1:20))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse lung cancer using PCK antibody and FNSA-0003 (at dilution of 1:20))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat Myocardium using alpha-SMA antibody and MBS7607160 (at dilution of 1:20))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat Myocardium using alpha-SMA antibody and MBS7607160 (at dilution of 1:20))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse breast cancer using PCNA antibody and FNSA-0003(at dilution of 1:30))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse breast cancer using PCNA antibody and FNSA-0003(at dilution of 1:30))
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Product Notes
The IgG (H+L) (Catalog #AAA7607160) is a Secondary Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG (H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Suggested dilution: 1:1000-1:10000 for western blotting with ECL substrates; 1:1000-1:20000 for ELISA and Western blotting with chromogenic substrates; 1:20-1:50 for IHC. Researchers should empirically determine the suitability of the IgG (H+L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H+L), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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