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3 results for "d108-specific-protein" - showing 1-3
12 kDa protein (ges), Recombinant Protein (Cat# AAA1173044)
Full Name
Recombinant Enterobacteria phage D108 12 kDa protein (ges)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,215/1 mg (E-Coli) | $1,770/1 mg (Yeast) | $2,815/1 mg (Baculovirus) | $1,415/0.1 mg (Baculovirus) | $2,035/0.1 mg (Mammalian-Cell) | $685/0.1 mg (E-Coli) | $900/0.1 mg (Yeast) | $5,680/0.5 mg (Mammalian-Cell) | $1,015/0.02 mg (Baculovirus) | $1,250/0.02 mg (Mammalian-Cell) | $590/0.02 mg (E-Coli) | $770/0.02 mg (Yeast)
Western Blot (WB)
(Figure 1. Western blot analysis of CD3 epsilon using anti-CD3 epsilon antibody (MBS1750783).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 epsilon antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3 epsilon at approximately 23KD. The expected band size for CD3 epsilon is at 23KD. )
CD3 epsilon, Polyclonal Antibody (Cat# AAA1750783)
Full Name
Anti-CD3 epsilon Picoband antibody
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
GES, Polyclonal Antibody (Cat# AAA7162426)
Full Name
Rabbit anti-Escherichia phage D108 (Bacteriophage D108) GES Polyclonal Antibody
Reactivity
Escherichia phage D108 (Bacteriophage D108)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
