Rabbit PPP1R1B Polyclonal Antibody | anti-PPP1R1B antibody

PPP1R1B, Phosphorylated (T75) (Protein Phosphatase 1 Regulatory subunit 1B, DARPP32, DARPP-32) (PE)

Cat. Num. AAA6432611

• Gene Names: PPP1R1B; DARPP32; DARPP-32
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence
• Purity: Purified by affinity chromatography.
• Synonyms: PPP1R1B; Polyclonal Antibody; Phosphorylated (T75) (Protein Phosphatase 1 Regulatory subunit 1B; DARPP32; DARPP-32) (PE); anti-PPP1R1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PPP1R1B, phosphorylated T75. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PPP1R1B antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:100-1:200; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding T75 of human PPP1R1B.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells, using Phospho-PPP1R1B-T75 antibody.)

Product Categories/Family for anti-PPP1R1B antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 335057524
• NCBI GeneID: 84152
• NCBI Accession #: NP_001229393.1
• NCBI GenBank Nucleotide #: NM_001242464.1
• UniProt Accession #: Q9UD71; Q547V9; Q547W0; Q9H7G1
• Molecular Weight: 22,963 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 1B isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 1B
• NCBI Official Symbol: PPP1R1B
• NCBI Official Synonym Symbols: DARPP32; DARPP-32
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B; dopamine and cAMP-regulated neuronal phosphoprotein 32
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R1B
• UniProt Synonym Gene Names: DARPP32
• UniProt Entry Name: PPR1B_HUMAN

NCBI Description

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell soma; cytoplasm; nucleus; cytosol

Molecular Function: protein kinase inhibitor activity; protein phosphatase type 1 regulator activity; D4 dopamine receptor binding; protein phosphatase inhibitor activity; D1 dopamine receptor binding; type 1 serine/threonine specific protein phosphatase inhibitor activity; D3 dopamine receptor binding; D2 dopamine receptor binding; D5 dopamine receptor binding

Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; regulation of catalytic activity; response to amphetamine; negative regulation of catalytic activity; visual learning; negative regulation of protein kinase activity; signal transduction