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16 results for " Axonal Guidance Proteins" - showing 1-16
Western Blot (WB)
(All lanes : Anti-DRAGON (RGMB) Antibody (C-term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: mouse brain lysateLane 3: mouse cerebellum lysateLane 4: NCI-H460 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
DRAGON, Polyclonal Antibody (Cat# AAA9216959)
Full Name
DRAGON (RGMB) Antibody (C-term)
Gene Names
RGMB; DRAGON
Reactivity
Human, Mouse
Applications
WB, IHC-P, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RGMB antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
RGMB, Polyclonal Antibody (Cat# AAA9132664)
Full Name
RGMB Polyclonal Antibody
Gene Names
RGMB; DRAGON
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS)
(Fig.1: MBS468051 BOSC23 cells were transiently transfected with an expression vector encoding either the ecd of human RGMB (red filled curve), or an irrelevant protein (control transfectant, black curve). Binding of BFH-5C9 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human RGMB.)
Repulsive Guidance Molecule B (RGMB), Monoclonal Antibody (Cat# AAA468051)
Full Name
Rat anti-human Repulsive Guidance Molecule B (RGMB) monoclonal antibody
Gene Names
RGMB; DRAGON
Reactivity
Human
Applications
FC/FACS, EIA, EIA
Purity
Protein G
Pricing
$550/0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry of RGM-B in mouse brain using MBS611754.)
RGMb, Polyclonal Antibody (Cat# AAA611754)
Full Name
RGMb (Repulsive Guidance Molecule B)
Gene Names
RGMB; DRAGON; FLJ90406; MGC86970; DKFZp434P228
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$835/0.1 mg | $3,750/5x0.1 mg
CRMP2, Polyclonal Antibody (Cat# AAA536402)
Full Name
CRMP2 antibody
Gene Names
DPYSL2; CRMP2
Applications
User optimized
Pricing
$385/0.1 mg | $1,730/5x0.1 mg
Western Blot (WB)
(Western blot analysis of GAP43 expression in SH-SY5Y celllysate.)
GAP43, Monoclonal Antibody (Cat# AAA179756)
Full Name
Anti-GAP43 Rabbit Monoclonal Antibody
Gene Names
GAP43; B-50; PP46
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-RGMB Antibody at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: 293 whole cell lysateLane 3: Human brain lysateLane 4: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
RGMB, Polyclonal Antibody (Cat# AAA9231098)
Full Name
RGMB Antibody
Gene Names
RGMB; DRAGON
Reactivity
Human, Mouse
Applications
WB, EIA
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Neuropilin 1 expression in mouse heart lysate.)
Neuropilin 1, Monoclonal Antibody (Cat# AAA179628)
Full Name
Anti-Neuropilin 1 Rabbit Monoclonal Antibody
Gene Names
NRP1; NP1; NRP; BDCA4; CD304; VEGF165R
Reactivity
Human, Mouse, Rat
Applications
FC, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of NCAM expression in SH-SY5Y cell lysate (MBS179171).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NCAM1 )
NCAM, Monoclonal Antibody (Cat# AAA179171)
Full Name
Anti-NCAM Rabbit Monoclonal Antibody
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of L1CAM expression in human fetal kidney lysate (MBS179429).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L1CAM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for L1CAM )
L1CAM, Monoclonal Antibody (Cat# AAA179429)
Full Name
Anti-L1CAM Rabbit Monoclonal Antibody
Gene Names
L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)
CD56/NCAM-1, ELISA Kit (Cat# AAA1751072)
Full Name
Monkey primate CD56/NCAM-1 PicoKine ELISA Kit
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$510/96-Strip-Wells | $2,305/5x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of DCAMKL1 expression in SH-SY5Y cell lysate; (MBS179935).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCLK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DCLK1 )
DCAMKL1, Monoclonal Antibody (Cat# AAA179935)
Full Name
Anti-DCAMKL1 Rabbit Monoclonal Antibody
Gene Names
DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SFRP1 expression in A375 cell lysate (MBS179778).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFRP1 )
SFRP1, Monoclonal Antibody (Cat# AAA179778)
Full Name
Anti-SFRP1 Rabbit Monoclonal Antibody
Gene Names
SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
Reactivity
Human
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (MBS1750310).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HELA whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40KD. The expected band size for CXCR4 is at 40KD. )
CXCR4, Polyclonal Antibody (Cat# AAA1750310)
Full Name
Anti-CXCR4 Picoband Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
WDFY3, Polyclonal Antibody (Cat# AAA9630048)
Full Name
WDFY3 Antibody
Gene Names
WDFY3; ALFY; ZFYVE25
Reactivity
Human, Mouse
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of DRAGON/RGMB using anti-DRAGON/RGMB antibody (MBS1753750).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human PC-3 whole cell lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DRAGON/RGMB antigen affinity purified polyclonal antibody (Catalog # MBS1753750) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DRAGON/RGMB at approximately 48KD. The expected band size for DRAGON/RGMB is at 48KD. )
DRAGON/RGMB, Polyclonal Antibody (Cat# AAA1753750)
Full Name
Anti-DRAGON/RGMB Antibody
Gene Names
RGMB; DRAGON
Reactivity
Human, Mouse
Applications
WB, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
