Western Blot (WB) (Western blot analysis of Int11 expression in SKOV3 (A), MCF7 (B), mouse spleen (C), rat liver (D) whole cell lysates.)
Immunofluorescence (IF) (Immunofluorescent analysis of Int11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Western Blot (WB) (Western blot analysis of CPSF4 Antibody (Center) in Jurkat cell line lysates (35ug/lane).CPSF4 (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CPSF3L antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using CPSF3L antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (CPSF3 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the CPSF3 antibody detected the CPSF3 protein (arrow).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CPSF3L antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using CPSF3L antibody. Blue: DAPI for nuclear staining.)
Testing Data (Gel: 8%SDS-PAGE Lysates (from left to right): Hela and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)
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