STAC2, NT (STAC2, SH3 and cysteine-rich domain-containing protein 2, 24b2/STAC2, Src homology 3 and cysteine-rich domain-containing protein 2) (MaxLight 750)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
UBASH3A, ID (UBASH3A, STS2, Ubiquitin-associated and SH3 domain-containing protein A, Cbl-interacting protein 4, Suppressor of T-cell receptor signaling 2, T-cell ubiquitin ligand 1) (MaxLight 650)
SHANK2, ID (SHANK2, CORTBP1, KIAA1022, PROSAP1, SH3 and multiple ankyrin repeat domains protein 2, Cortactin-binding protein 1, Proline-rich synapse-associated protein 1) (MaxLight 750)
UBASH3A, ID (UBASH3A, STS2, Ubiquitin-associated and SH3 domain-containing protein A, Cbl-interacting protein 4, Suppressor of T-cell receptor signaling 2, T-cell ubiquitin ligand 1) (Azide free) (HRP)
PACSIN3 (Protein Kinase C and Casein Kinase Substrate in Neurons 3, PACSIN-3, Endophilin 1, Endophilin I, Endophilin-I, SH3 Domain-containing Protein 6511) (MaxLight 405)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
BIN 1 (Bridging Integrator 1, Amphiphysin II, Amphiphysin-like Protein, Box-dependent Myc-interacting Protein 1, Myc Box-dependent Interacting Protein 1) (MaxLight 490)
SAMSN1, ID (SAMSN1, HACS1, SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1, Nash1, SAM domain, SH3 domain and nuclear localization signals protein 1, SH3-SAM adaptor protein) (MaxLight 750)
Gene Names
SAMSN1; SLy2; HACS1; NASH1; SASH2; SH3D6B
Reactivity
Human
Applications
IHC, WB, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Western Blot (WB) (Western Blot analysis of STAM2 expression in transfected 293T cell line by STAM2 polyclonal antibody. Lane 1: STAM2 transfected lysate (58.2kD). Lane 2: Non-transfected lysate. )
Western Blot (WB) (Western blot analysis of RUSC1 expression in A549 (A), mouse brain (B), rat lung (C) whole cell lysates.)
Western Blot (WB) (Western blot analysis of SNX9 expression in HeLa (A), mouse liver (B), rat heart (C) whole cell lysates.)
Western Blot (WB) (Western blot analysis of WBP11 expression in HEK293T (A), mouse brain (B) whole cell lysates.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SH3GL2 antibody.)
Western Blot (WB) (Western blot analysis of Hela cell lysate using SNX9 antibody.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SH3GL2 antibody.)
Western Blot (WB) (Host: RabbitTarget Name: NCF1Sample Tissue: Human 293T Whole Cell lysatesAntibody Dilution: 1ug/ml)
Western Blot (WB) (Western blot analysis of Hela cell lysate using SAMSN1 Polyclonal Antibody at dilution of 1:800)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using SAMSN1 Polyclonal Antibody at dilution of 1:50(×200))
Testing Data (Detection limit for recombinant GST tagged NCF4 is ~0.3ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using Phospho-G3BP1-S232 antibody.)
Western Blot (WB) (SH3KBP1 monoclonal antibody (M01), clone 1F11. Western Blot analysis of SH3KBP1 expression in IMR-32.)
Western Blot (WB) (Host: RabbitTarget Name: SH3D19Sample Type: PANC1 Whole Cell lysatesAntibody Dilution: 1.0ug/mlSH3D19 is supported by BioGPS gene expression data to be expressed in PANC1)
Western Blot (WB) (Western blot analysis of SH3BGRL Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in human Uterus tissue lysate. SH3BGRL Antibody (Center) (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western Blot detection against Immunogen (37kD).)
Western Blot (WB) (Western Blot analysis of RBM12 expression in transfected 293T cell line by RBM12 monoclonal antibody Lane 1: RBM12 transfected lysate (Predicted MW: 97.4kD). Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged RBM12 is 0.03ng/ml as a capture antibody.)
Western Blot (WB) (UBASH3A Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the UBASH3A antibody detected the UBASH3A protein (arrow).)
Western Blot (WB) (Western Blotting Analysis: Representative lot data. Mouse brain tissue lysate was probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)
Western Blot (WB) (Western Blotting Analysis Representative lot data: Rat brain (lane 1) and mouse brain (lane 2) membrane tissue lysates were probed with Anti-Endophilin-A1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates Endophilin-A1 (~42kD). Note: Film exposure time: 15 min.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells using G3BP-1(Ab-232) Antibody and the same antibody preincubated with blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Ab-232) Antibody(left) or the same antibody preincubated with blocking peptide(right).)
Western Blot (WB) (Western Blotting Analysis: Representative lot data. Mouse brain tissue lysate was probed with Anti-Amphiphysin II (1:1000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrows indicate Amphiphysin I and II (~105kD, 75kD).)
Western Blot (WB) (Western Blotting Analysis: Representative lot data. Rat brain membrane (lane 1) and mouse brain membrane (lane 2) tissue lysates were probed with Anti-Amphiphysin II (1:1000 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrows indicate Amphiphysin I and II (~105kD, 75kD).)
Western Blot (WB) (Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6001563. SAMSN1 (arrow) was detected usingMBS6001563.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma using MBS6001563 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001563 for immunohistochemistry.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Jurkat cells using MBS6001563 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Testing Data (NG108-15 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS603651 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SETA (85kD).)
Western Blot (WB) (Western blot analysis of anti-Endophilin B1 Antibody (Y80) in HepG2 cell line lysates (35ug/lane). SH3GLB1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Vinexin (SORBS3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.)
Testing Data (NG108-15 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SETA, (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SETA (85kD).)
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