Testing Data (Figure 1. Response of ACTOne GPBAR1 (TGR5) cell line & parental cell line to LCA. ACTOne GPBAR1 cells and parental cells were plated overnight in 20 ul culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ul/well of 1X Dye-loading solution (Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of LCA. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of LCA in ACTOne TGR5 cell line. EC50 = 3.2 uM in the presence of PDE inhibitor. B. Parental cells do not respond to LCA)
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