The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 () Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.01ug/ml, 0.1ug/ml, 0.001ug/ml and 0.003ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Flow Cytometry (FC/FACS) (Flow-cytometry using the anti-Podoplanin (MAP tag) antibody PMab-1 (MBS488581) NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)