$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
Applications
WB, IHC-P
Purity
Affinity purified by Protein A
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NPFFR2 antibody.)
Western Blot (WB) (NPFFR1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NPFFR1 antibody detected the NPFFR1 protein (arrow).)
Western Blot (WB) (Host: RabbitTarget Name: NPFFR1Sample Tissue: Human Hela Whole Cell lysatesAntibody Dilution: 1ug/ml)
Immunohistochemistry (IHC) (IHC image of MBS7134347 diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Western Blot (WB) (Host: RabbitTarget Name: NPFFR1Sample Tissue: Human Jurkat Whole Cell lysatesAntibody Dilution: 1ug/ml)
Western Blot (WB) (WB Suggested Anti-NPFFR2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate)
Western Blot (WB) (NPFFR1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NPFFR1 antibody detected the NPFFR1 protein (arrow).)
Western Blot (WB) (NPFFR2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NPFFR2 antibody detected the NPFFR2 protein (arrow).)
Western Blot (WB) (NPFFR1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NPFFR1 antibody detected the NPFFR1 protein (arrow).)
Western Blot (WB) (NPFFR1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NPFFR1 antibody detected the NPFFR1 protein (arrow).)
Western Blot (WB) (NPFFR2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NPFFR2 antibody detected the NPFFR2 protein (arrow).)
Immunofluorescence (IF) (Immunofluorescence staining of U251 cells with MBS7134922 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Western Blot (WB) (NPFFR1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NPFFR1 antibody detected the NPFFR1 protein (arrow).)
Western Blot (WB) (NPFFR2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NPFFR2 antibody detected the NPFFR2 protein (arrow).)
Western Blot (WB) (NPFFR2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NPFFR2 antibody detected the NPFFR2 protein (arrow).)
Immunohistochemistry (IHC)-Paraffin ( Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (bs-12018R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Western Blot (WB) (Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-NPFF1 Receptor (bs-12018R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD )
Immunohistochemistry (IHC)-Paraffin (Dilution: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (bs-12018R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.