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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant HSD17B2 protein (red) or empty vector (blue) stained using HSD17B2 antibody)

Mouse HSD17B2 Monoclonal Antibody | anti-HSD17B2 antibody

HSD17B2 antibody

Gene Names
Hsd17b2; AI194836; AI194967; AI255511
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
HSD17B2 antibody was purified by affinity chromatography.
Synonyms
HSD17B2; Monoclonal Antibody; HSD17B2 antibody; Monoclonal HSD17B2; Anti-HSD17B2; EDH17B2; HSD17; SDR9C2; HSDB2 17; HSDB2-17; anti-HSD17B2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4F10
Purity/Purification
HSD17B2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
227
Applicable Applications for anti-HSD17B2 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
FC: 1:100
WB: 1:2000
Biological Significance
This protein is capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
Cross-Reactivity
Human
Immunogen
HSD17B2 antibody was raised in Mouse using full length human recombinant protein of human HSD17B2 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant HSD17B2 protein (red) or empty vector (blue) stained using HSD17B2 antibody)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant HSD17B2 protein (red) or empty vector (blue) stained using HSD17B2 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSD17B2 protein (Right) or empty vector (Left) detected with HSD17B2 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSD17B2 protein (Right) or empty vector (Left) detected with HSD17B2 antibody)
Related Product Information for anti-HSD17B2 antibody
Mouse monoclonal HSD17B2 antibody
Product Categories/Family for anti-HSD17B2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,836 Da
NCBI Official Full Name
Hsd17b2 protein
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 2
NCBI Official Symbol
Hsd17b2
NCBI Official Synonym Symbols
AI194836; AI194967; AI255511
NCBI Protein Information
estradiol 17-beta-dehydrogenase 2
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 2
UniProt Gene Name
Hsd17b2
UniProt Synonym Gene Names
Edh17b2; 17-beta-HSD 2
UniProt Entry Name
DHB2_MOUSE

Uniprot Description

HSD17B2: Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Lipid Metabolism - androgen and estrogen; Endoplasmic reticulum; Oxidoreductase; EC 1.1.1.239; EC 1.1.1.62; Membrane protein, integral

Cellular Component: membrane; intracellular membrane-bound organelle; integral to membrane

Molecular Function: estradiol 17-beta-dehydrogenase activity; oxidoreductase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity

Biological Process: response to retinoic acid; metabolic process; in utero embryonic development; androgen biosynthetic process; lipid metabolic process; estrogen biosynthetic process; steroid biosynthetic process; placenta development

Research Articles on HSD17B2

Similar Products

Product Notes

The HSD17B2 hsd17b2 (Catalog #AAA832827) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSD17B2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). FC: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the HSD17B2 hsd17b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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