Western Blot (WB) (Western Blot analysis of Phospho-PKC alpha (T638) on different lysates using anti-Phospho-PKC alpha (T638) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293)
Immunocytochemistry (ICC) (ICC staining Phospho-PKC alpha (T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using MBS7124158(PRKCA Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: ×200))
Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using MBS7124158(PRKCA Antibody) at dilution 1/10, on the right is treated with fusion protein. (Original magnification: ×200))
SDS-Page (Gel: 10%SDS-PAGE, Lysate: 50 ug, Lane 1-2: Jurkat cells, HT29 cells, Primary antibody: MBS7124158(PRKCA Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CLU antibody.)
Western Blot (WB) (Western Blot detection against Immunogen (34.43kD).)
Western Blot (WB) (EIF4G2 monoclonal antibody. Western Blot analysis of EIF4G2 expression in PC-12.)
Western Blot (WB) (EIF4G2 monoclonal antibody Western Blot analysis of EIF4G2 expression in HeLa NE.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to EIF4G2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)
Western Blot (WB) (Western blot analysis of OPRS1 (arrow) using rabbit polyclonal OPRS1 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OPRS1 gene (Lane 2) (Origene Technologies).)
Western Blot (WB) (OPRS1 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the OPRS1 antibody detected the OPRS1 protein (arrow).)
Western Blot (WB) (CHCHD2 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CHCHD2 antibody detected the CHCHD2 protein (arrow).)
Immunohistochemistry (IHC) (CHCHD2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHCHD2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (CHCHD2 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of OPRS1 (arrow) using rabbit polyclonal OPRS1 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OPRS1 gene (Lane 2) (Origene Technologies).)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of NCI-H292 cells using OPRS1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of OPRS1 (arrow) using rabbit polyclonal OPRS1 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the OPRS1 gene (Lane 2) (Origene Technologies).)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of NCI-H292 cells using OPRS1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (CHCHD2 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CHCHD2 antibody detected the CHCHD2 protein (arrow).)
Immunohistochemistry (IHC) (CHCHD2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHCHD2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (CHCHD2 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of PRKCA Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). PRKCA (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (PRKCA Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of PRKCA Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB) (Western blot analysis of PRKCA Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). PRKCA (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (PRKCA Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of PRKCA Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB) (Western blot analysis of CEM cell line lysates (35ug/lane). using. GAPDH (arrow) was detected using)
Testing Data (All lanes : at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20ug/ lane. Secondary IgG (HRP) GtxMoPredicted band size : 36 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (All lanes : at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20ug/ lane. Secondary IgG (HRP) GtxMoPredicted band size : 36 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H.kidney section using at 1:25 dilution. IgG (HRP) GtxMo was used as the secondary antibody, followed by DAB staining.)
Testing Data (A431 (Lane1), C2C12 lysate (L2), HEK293 lysate (L3), Hela lysate (L4), HepG2 lysate (L5), Human Placenta lysate (L6), Huvec lysate (L7) and L6 lysate (L8) were resolved by electrophoresis, transferred to PVDF membrane and probed with G8140-01 (1:500). Proteins were visualized using IgG (HRP) goat anti- mouse and an ECL detection system. Arrow indicates Glyceraldehyde-3-PDH (~38kD).)
Testing Data (Confolcal fluorescent analysis of HeLa and NIH/3T3 cells using G8140-01 (Red). Actin filaments have been labeled with Alexa Fluor 488 - Phalloidin (Green). Nuclear is stained with DAPI (Blue). Cytosol staining.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of PKC alpha Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS) (PKC alpha Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of anti-PKCalpha Pab in placenta lysate. PKCalpha (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (Western blot analysis of CEM cell line lysates (35ug/lane). using. GAPDH (arrow) was detected using)
Testing Data (All lanes : at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20ug/ lane. Secondary IgG (HRP) GtxMoPredicted band size : 36 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (All lanes : at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20ug/ lane. Secondary IgG (HRP) GtxMoPredicted band size : 36 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H.kidney section using at 1:25 dilution. IgG (HRP) GtxMo was used as the secondary antibody, followed by DAB staining.)
Immunofluorescence (IF) (Immunofluorescence of Hela cells stained with at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red))
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB) (Western blot analysis of CEM cell line lysates (35ug/lane). using. GAPDH (arrow) was detected using)
Testing Data (All lanes : at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20ug/ lane. Secondary IgG (HRP) GtxMoPredicted band size : 36 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (All lanes : at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20ug/ lane. Secondary IgG (HRP) GtxMoPredicted band size : 36 kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H.kidney section using at 1:25 dilution. IgG (HRP) GtxMo was used as the secondary antibody, followed by DAB staining.)
Immunofluorescence (IF) (Immunofluorescence of Hela cells stained with at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red))
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB) (Western blot analysis of extracts from various samples, using Lamin A/C Antibody.Lane 1: MCF7 cells(UV treatment), blocked with antigen-specific peptides,Lane 2: MCF7 cells(UV treatment),Lane 3: Hela cells(LPS treatment),Lane 4: Hela cells(heat shock treatment).)
Immunofluorescence (IF)/Immunocytochemistry (ICC) (Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6752) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PRKCA antibody.)
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.