Western Blot (WB) (Western blot analysis of KAP1 expression in HeLa cell lysate (MBS179314).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM28 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRIM28 )
Western Blot (WB) (Western blot analysis KAP1 using 293 whole cell lysates)
SDS-Page
Western Blot (WB) (Western blot analysis of TIF1 beta expression in HT29 (A), SKOV3 (B), mouse brain (C) whole cell lysates.)
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