Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Goat IgG Fc " - showing 7700-7750
Western Blot (WB)
(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS649023. JUP (arrow) was detected using the purified Pab.)
Catenin, gamma, Polyclonal Antibody (Cat# AAA6467290)
Full Name
Catenin, gamma, CT (Catenin (Cadherin-associated Protein) gamma (80kD), CTNNG, ARVD12, Desmoplakin 3, Desmoplakin-3, DP3, Desmoplakin III, Desmoplakin-III, DPIII, Gamma Catenin, Junction Plakoglobin, JUP, PDGB, PKGB) (MaxLight 490)
Gene Names
JUP; DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12
Reactivity
Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PCSK9 antibody (C-term) in Hela cell line lysates (35ug/lane). PCSK9 (arrow) was detected using the purified Pab.)
Proprotein Convertase Subtilisin Kexin 9, Polyclonal Antibody (Cat# AAA6494715)
Full Name
Proprotein Convertase Subtilisin Kexin 9, CT (Proprotein Convertase Subtilisin/kexin Type 9, PCSK9, FH3, Hypercholesterolemia Autosomal Dominant 3, HCHOLA3, LDLCQ1, Neural Apoptosis Regulated Convertase 1, NARC1, NARC-1, PC9, Proprotein Convertase PC9, PS
Gene Names
PCSK9; FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
WB, IHC
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane) usingMBS6007185. This demonstrates that MBS6007185 detected the RARA protein (arrow).)
RARA, Polyclonal Antibody (Cat# AAA6340314)
Full Name
RARA, CT (RARA, NR1B1, Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1) (MaxLight 650)
Gene Names
RARA; RAR; NR1B1
Reactivity
Human, Mouse
Applications
IHC, WB, IF, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Western Blot (WB)
(SLC36A1 Antibody (N-term) western blot analysis in NCI-H460(lane 1),K562(lane 2),A549(lane 3) cell line lysates (35ug/lane).This demonstrates the SLC36A1 antibody detected the SLC36A1 protein (arrow).)
SLC36A1, Polyclonal Antibody (Cat# AAA6348376)
Full Name
SLC36A1, NT (SLC36A1, PAT1, Proton-coupled amino acid transporter 1, Solute carrier family 36 member 1) (Biotin)
Gene Names
SLC36A1; Dct1; PAT1; LYAAT1; TRAMD3
Reactivity
Human
Applications
FC/FACS, EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of 033516 in CEM cell line lysates (35ug/lane). CD46 (arrow) was detected using the purified Pab.)
CD46, Polyclonal Antibody (Cat# AAA6283283)
Full Name
CD46, CT (CD46, MCP, MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46) (FITC)
Gene Names
CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
Reactivity
Human
Applications
FC/FACS, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(GCNT3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).)
GCNT3, Polyclonal Antibody (Cat# AAA6301708)
Full Name
GCNT3, CT (GCNT3, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase) (PE)
Gene Names
GCNT3; GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
Reactivity
Human, Mouse
Applications
FC/FACS, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(MLM Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MLM antibody detected the MLM protein (arrow).)
MLM, Polyclonal Antibody (Cat# AAA6321178)
Full Name
MLM, CT (CDKN2A, CDKN2, MLM, Cyclin-dependent kinase inhibitor 2A, isoform 4, p14ARF, p19ARF) (Azide free) (HRP)
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western blot analysis of PAX6 Antibody (Center) in Hela cell line lysates (35ug/lane). PAX6 (arrow) was detected using the purified Pab.)
Pax6, Polyclonal Antibody (Cat# AAA6492269)
Full Name
Pax6 (Paired Box Gene 6 (Aniridia, Keratitis), Paired Box Homeotic Gene 6, Paired Box Protein Pax-6, Pax-6, Aniridia Type II Protein, AN2, AN, D11S812E, MGC17209, MGDA, Oculorhombin, WAGR) (PE)
Gene Names
PAX6; AN; AN2; MGDA; WAGR; D11S812E
Reactivity
Human
Applications
WB
Purity
Purified by Immunoaffinity chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Glucagon Antibody (C-term) in Jurkat cell line lysates (35ug/lane). GCG (arrow) was detected using the purified Pab.)
Glucagon, Polyclonal Antibody (Cat# AAA6477917)
Full Name
Glucagon, CT (GCG, Glicentin-related Polypeptide, GRPP) (AP)
Gene Names
GCG; GLP1; GLP2; GRPP; GLP-1
Reactivity
Human
Applications
FC/FACS, EIA, IF, WB, IHC
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) using E3224-02A: Lane 1: nontransfected Lane 2: transiently transfected with the ENPP2 gene.)
ENPP2, Polyclonal Antibody (Cat# AAA6474053)
Full Name
ENPP2, ID (Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, E-NPP 2, Extracellular Lysophospholipase D, LysoPLD, Autotaxin, ATX, PDNP2) (MaxLight 490)
Gene Names
ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
Reactivity
Human
Applications
FC/FACS, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ATP5A1 on different cells lysates using anti-ATP5A1 antibody at 1/500 dilution. Positive control�� Line 1: Hela Line 2: MCF-7)
ATP5A1, Monoclonal Antibody (Cat# AAA9436211)
Full Name
ATP5A1 Antibody
Gene Names
ATP5A1; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1, 000 dilution.)
hUPF1, Monoclonal Antibody (Cat# AAA9436675)
Full Name
hUPF1 Antibody
Gene Names
UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1
Reactivity
Human, Mouse
Applications
WB, ICC, IF, IP, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western Blot (WB) analysis of USP9x Rabbit mAb at 1:1000 dilutionLane1:SiHa whole cell lysateLane2:A549 whole cell lysateLane3:293 whole cell lysateLane4:The colon tissue lysate of Mouse)
USP9x, Polyclonal Antibody (Cat# AAA3014170)
Full Name
USP9x Polyclonal Antibody
Gene Names
USP9X; FAF; FAM; DFFRX; MRX99; MRXS99F
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC/FACS
Purity
Protein A affinity purified.
Pricing
$355/0.05 mL | $470/0.1 mL | $1,855/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in MCF7 cell line lysates (35ug/lane) usingMBS625868. BMI1 (arrow) was detected usingMBS625868.)
BMI1, Polyclonal Antibody (Cat# AAA6465442)
Full Name
BMI1 (Polycomb Complex Protein BMI-1, Polycomb Group RING Finger Protein 4, RING Finger Protein 51, BMI1, PCGF4, RNF51) (FITC)
Gene Names
BMI1; PCGF4; RNF51; FLVI2/BMI1
Reactivity
Human, Mouse
Applications
IHC, FC/FACS, IF, WB
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(The Presenilin 1 (PSEN1) Antibody (C-term) is used in Western blot to detect PSEN1 in mouse kidney tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.)
Presenilin 1, Polyclonal Antibody (Cat# AAA6494252)
Full Name
Presenilin 1, CT (Presenilin-1, PS-1, Protein S182, Presenilin-1 NTF Subunit, Presenilin-1 CTF Subunit, Presenilin-1 CTF12, PS1-CTF12, AD3, PS1, PSNL1, PSEN1) (Azide free) (HRP)
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies).)
ERK1, Polyclonal Antibody (Cat# AAA6474823)
Full Name
ERK1, NT (Extracellular Signal-regulated Kinase 1, ERK-1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, MAPK3, Mitogen-activated Protein Kin
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies).)
ERK1, Polyclonal Antibody (Cat# AAA6474833)
Full Name
ERK1, NT (Extracellular Signal-regulated Kinase 1, ERK-1, ERT2, Insulin-stimulated MAP2 Kinase, MAP Kinase Isoform p44, Microtubule-associated Protein 2 Kinase, Mitogen-activated Protein Kinase 3, MAP Kinase 3, MAPK 3, MAPK3, Mitogen-activated Protein Kin
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-CSF1R Pab in human placenta. CSF1R (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
CD115, Polyclonal Antibody (Cat# AAA6468193)
Full Name
CD115, CT (CD115 Antigen, c-fms, CFMS, Colony Stimulating Factor 1 Receptor, Colony Stimulating Factor I Receptor, CSF 1 R, CSF-1-R, CSF1R, CSFR, FIM2, FMS Proto-oncogene, Macrophage Colony Stimulating Factor 1 Receptor, MCSF Receptor, Proto-oncogene c-Fm
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(TIMP1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TIMP1 antibody detected the TIMP1 protein (arrow).)
TIMP1, Polyclonal Antibody (Cat# AAA6355631)
Full Name
TIMP1, CT (TIMP1, CLGI, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1) (MaxLight 550)
Gene Names
TIMP1; EPA; EPO; HCI; CLGI; TIMP; TIMP-1
Reactivity
Human
Applications
FC/FACS, IF, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(UGT2B15 Antibody (Center) western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).This demonstrates the UGT2B15 antibody detected the UGT2B15 protein (arrow).)
UGT2B15, Polyclonal Antibody (Cat# AAA6361189)
Full Name
UGT2B15, ID (UGT2B15, UGT2B8, UDP-glucuronosyltransferase 2B15, HLUG4, UDP-glucuronosyltransferase 2B8, UDPGTh-3) (PE)
Gene Names
UGT2B15; HLUG4; UGT2B8; UDPGTH3; UDPGT 2B8; UDPGT2B15
Reactivity
Human
Applications
FC/FACS, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ST6 (arrow) usingMBS646569. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD82 gene (Lane 2) (Origene Technologies).)
CD82, Polyclonal Antibody (Cat# AAA6283511)
Full Name
CD82, CT (CD82, KAI1, SAR2, ST6, TSPAN27, CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, CD82) (AP)
Gene Names
CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by Protein A/G Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of EIF2AK4 using anti-EIF2AK4 antibody (M01172).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EIF2AK4. )
GCN2, Monoclonal Antibody (Cat# AAA1752063)
Full Name
Anti-GCN2 Monoclonal Antibody
Gene Names
EIF2AK4; GCN2; PVOD2
Reactivity
Human
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of POR using anti-POR antibody (M02166).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POR. )
Cytochrome P450 Reductase, Monoclonal Antibody (Cat# AAA1752176)
Full Name
Anti-Cytochrome P450 Reductase Monoclonal Antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CYP27B1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). CYP27B1 (arrow) was detected using the purified Pab.)
CYP27B1, Polyclonal Antibody (Cat# AAA6290465)
Full Name
CYP27B1, CT (CYP27B1, CYP1ALPHA, CYP27B, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome
Gene Names
CYP27B1; VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha
Reactivity
Human
Applications
FC/FACS, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western Blot analysis in mouse spleen tissue lysates (35ug/lane) usingMBS648513. This demonstrates that MBS648513 detected SLC9A9 protein (arrow).)
SLC9A9, Polyclonal Antibody (Cat# AAA6348585)
Full Name
SLC9A9, CT (SLC9A9, NHE9, Sodium/hydrogen exchanger 9, Na(+)/H(+) exchanger 9, Solute carrier family 9 member 9) (Biotin)
Gene Names
SLC9A9; NHE9; AUTS16
Reactivity
Human, Mouse
Applications
FC/FACS, EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(NUDT15 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NUDT15 antibody detected the NUDT15 protein (arrow).)
NUDT15, Polyclonal Antibody (Cat# AAA6327118)
Full Name
NUDT15, CT (NUDT15, MTH2, Probable 8-oxo-dGTP diphosphatase NUDT15, 7,8-dihydro-8-oxoguanine-triphosphatase NUDT15, MutT homolog 2, Nucleoside diphosphate-linked moiety X motif 15) (APC)
Gene Names
NUDT15; MTH2
Reactivity
Human, Mouse
Applications
FC/FACS, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IDH1 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IDH, Polyclonal Antibody (Cat# AAA6483638)
Full Name
Isocitrate Dehydrogenase [NADP], Cytoplasmic (IDH, IDCD, IDH1, IDP, IDPC, Cytosolic NADP-isocitrate Dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) (HRP)
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human, Mouse
Applications
WB, EIA, IHC
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of IDH1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IDH, Polyclonal Antibody (Cat# AAA6483646)
Full Name
Isocitrate Dehydrogenase [NADP], Cytoplasmic, NT (IDH, IDCD, IDH1, IDP, IDPC, Cytosolic NADP-isocitrate Dehydrogenase, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) (APC)
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western blot analysis of AMPK alpha 1 on different lysates using anti-AMPK alpha 1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: MCF-7)
AMPK alpha 1, Monoclonal Antibody (Cat# AAA9435648)
Full Name
AMPK alpha 1 Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CYP27A1 on different tissue lysates using anti-CYP27A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat heart Lane 2: Human liver Lane 2: Human kidney)
CYP27A1, Monoclonal Antibody (Cat# AAA9436705)
Full Name
CYP27A1 Antibody
Gene Names
CYP27A1; CTX; CP27; CYP27
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CUL1 using anti-CUL1 antibody (M00658).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUL1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CUL1. )
Cullin 1, Monoclonal Antibody (Cat# AAA1752002)
Full Name
Anti-Cullin 1 Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IGFBP7 using anti-IGFBP7 antibody (M02410).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP7 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP7. )
IGFBP7, Monoclonal Antibody (Cat# AAA1752206)
Full Name
Anti-IGFBP7 Monoclonal Antibody
Gene Names
IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
Pericentriolar Material 1 Protein, Polyclonal Antibody (Cat# AAA6492685)
Full Name
Pericentriolar Material 1 Protein (PCM1, PCM-1, hPCM-1, PTC4) (MaxLight 405)
Gene Names
PCM1; PTC4
Reactivity
Human
Applications
WB
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with NPM1 Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.)
NPM1, Polyclonal Antibody (Cat# AAA6490725)
Full Name
NPM1, CT (Nucleophosmin, NPM, Nucleolar Phosphoprotein B23, Numatrin, Nucleolar Protein NO38, NPM) (MaxLight 750)
Gene Names
NPM1; B23; NPM
Reactivity
Human
Applications
WB
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western Blot analysis using MBS646213 in A549 cell line lysates (35ug/lane).This demonstrates the CLU antibody detected the CLU protein (arrow).)
CLU, Polyclonal Antibody (Cat# AAA6286989)
Full Name
CLU, NT (CLU, APOJ, CLI, KUB1, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, Clusterin beta
Gene Names
CLU; CLI; AAG4; APOJ; KUB1; SGP2; SGP-2; SP-40; TRPM2; TRPM-2; MGC24903; CLU
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western Blot analysis using MBS646213 in A549 cell line lysates (35ug/lane).This demonstrates the CLU antibody detected the CLU protein (arrow).)
CLU, Polyclonal Antibody (Cat# AAA6286996)
Full Name
CLU, NT (CLU, APOJ, CLI, KUB1, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, Clusterin beta
Gene Names
CLU; CLI; AAG4; APOJ; KUB1; SGP2; SGP-2; SP-40; TRPM2; TRPM-2; MGC24903; CLU
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: Jurkat)
Rho A+B+C, Monoclonal Antibody (Cat# AAA9435489)
Full Name
Rho A+B+C Antibody
Gene Names
RHOC; H9; ARH9; ARHC; RHOH9
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CACNA2D2 using anti-CACNA2D2 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA2D2 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CACNA2D2 at approximately 130KD. The expected band size for CACNA2D2 is at 130KD.)
CACNA2D2, Polyclonal Antibody (Cat# AAA1751357)
Full Name
Anti-CACNA2D2 Antibody
Gene Names
CACNA2D2; CACNA2D
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ISL1 using anti-ISL1 antibody (M02969-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISL1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ISL1. )
Islet1, Monoclonal Antibody (Cat# AAA1752247)
Full Name
Anti-Islet1 Monoclonal Antibody
Gene Names
ISL1; Isl-1; ISLET1
Reactivity
Human
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of SKP2 using anti-SKP2 antibody (M00544).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SKP2. )
SKP2, Monoclonal Antibody (Cat# AAA1751978)
Full Name
Anti-SKP2 Monoclonal Antibody
Gene Names
SKP2; p45; FBL1; FLB1; FBXL1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of DDX5 using anti-DDX5 antibody (M00670).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX5 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDX5. )
DDX5, Monoclonal Antibody (Cat# AAA1752004)
Full Name
Anti-DDX5 Monoclonal Antibody
Gene Names
DDX5; p68; HLR1; G17P1; HUMP68
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of SIN3A using anti-SIN3A antibody (M01203).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIN3A antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SIN3A. )
mSin3A, Monoclonal Antibody (Cat# AAA1752070)
Full Name
Anti-mSin3A Monoclonal Antibody
Gene Names
SIN3A; WITKOS
Reactivity
Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ALCAM using anti-ALCAM antibody (M01788).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALCAM antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALCAM. )
CD166, Monoclonal Antibody (Cat# AAA1752135)
Full Name
Anti-CD166 Monoclonal Antibody
Gene Names
ALCAM; MEMD; CD166
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of UBE3A Antibody (C-term) in T47D cell line lysates (35ug/lane).UBE3A(arrow) was detected using the purified Pab.)
UBE3A, Polyclonal Antibody (Cat# AAA6473440)
Full Name
UBE3A, CT (Ubiquitin-protein Ligase E3A, E6AP Ubiquitin-protein Ligase, Oncogenic Protein-associated Protein E6-AP, Human Papillomavirus E6-associated Protein, Renal Carcinoma Antigen NY-REN-54, E6AP, EPVE6AP, HPVE6A) (PE)
Gene Names
UBE3A; AS; ANCR; E6-AP; HPVE6A; EPVE6AP; FLJ26981; UBE3A
Reactivity
Human
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of EEF1A1 Antibody (N-term) in Y79, T47D cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
EEF1A1, Polyclonal Antibody (Cat# AAA6473552)
Full Name
EEF1A1, NT (Elongation Factor 1-alpha 1, EF-1-alpha-1, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1 A-1, eEF1A-1, Elongation Factor Tu, EF-Tu, Leukocyte Receptor Cluster Member 7, LENG7, EF1A, EEF1A) (AP)
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Human
Applications
WB, EIA
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western Blot analysis using MBS646213 in A549 cell line lysates (35ug/lane).This demonstrates the CLU antibody detected the CLU protein (arrow).)
CLU, Polyclonal Antibody (Cat# AAA6286995)
Full Name
CLU, NT (CLU, APOJ, CLI, KUB1, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, Clusterin beta
Gene Names
CLU; CLI; AAG4; APOJ; KUB1; SGP2; SGP-2; SP-40; TRPM2; TRPM-2; MGC24903; CLU
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of TBPL1 using anti-TBPL1 antibody (M07468).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBPL1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TBPL1. )
TBPL1, Monoclonal Antibody (Cat# AAA1752382)
Full Name
Anti-TBPL1 Monoclonal Antibody
Gene Names
TBPL1; TLF; TLP; STUD; TRF2; MGC:8389; MGC:9620
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of MTA2 using anti-MTA2 antibody (M03073).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTA2. )
MTA2, Monoclonal Antibody (Cat# AAA1752251)
Full Name
Anti-MTA2 Monoclonal Antibody
Gene Names
MTA2; PID; MTA1L1
Reactivity
Human
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of EEF2 using anti-EEF2 antibody (M00830-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EEF2. )
EEF2, Monoclonal Antibody (Cat# AAA1752024)
Full Name
Anti-EEF2 Monoclonal Antibody
Gene Names
EEF2; EF2; EF-2; EEF-2; SCA26
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of LGALS1 using anti-LGALS1 antibody (M00470-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGALS1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LGALS1. )
Galectin 1, Monoclonal Antibody (Cat# AAA1751964)
Full Name
Anti-Galectin 1 Monoclonal Antibody
Gene Names
LGALS1; GBP; GAL1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
