Western Blot (WB) (All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilutionLane 1: mouse thymus lysatesLane 2: rat spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western blot analysis of SOCS1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOCS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) with 293 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS) (SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204783 PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Western Blot (WB) (Western blot analysis of PI3KC3 (S34) in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S34), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded H.liver tissue reacted with PI3KC3 Antibody (S34) (Cat#MBS9204783).)
Western Blot (WB) (Western blot analysis of extracts of Mouse brain tissue sample, using NuaK1 Antibody(MBS9603198).)
Western Blot (WB) (Western blot analysis GCVK_HHV7J using K562 whole cell lysates)
Western Blot (WB) (Western blot analysis of extracts of Human brain tissue sample, using QIK/QSK Antibody(MBS9603201).)
Western Blot (WB) (Western blot analysis of CKIP-1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CKIP-1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CKIP-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of 293 cells using CKIP-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis on Jurkat cell lysate using C1orf57 Antibody)
Immunofluorescene (IF) (MBS9600349 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts of mouse adrenal tissue sample, using SIK Antibody(MBS9603203).)
Western Blot (WB) (Western blot detection of Skp1 in Jurkat, A549, MCF7, C6 and Hela cell lysates using Skp1 mouse mAb (1:5000 diluted).Predicted band size:19KDa.Observed band size:19KDa.)
Western Blot (WB) (Western blot analysis using SKP1 mouse mAb against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Hela cells using SKP1 mouse mAb (green) and negative control (red).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 mouse mAb with DAB staining.)
Western Blot (WB) (Western blot analysis of lysates from 293, mouse NIH/3T3, rat C6 cell line and mouse kidney tissue lysate(from left to right), using Mouse Cdk9 Antibody (C-term). MBS9208988 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB) (Western blot analysis of extracts from Jurkat, A549, MCF7, C6 and Hela cell lysates using SQSTM1/p62 mouse mAb (1:1000 diluted).Predicted band size:62KDa.Observed band size:62KDa.)
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