The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot analysis of KCNN4 expression in K562 (A), HepG2 (B), mouse brain (C), rat brain (D) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of KCNN4 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Western Blot (WB) (Western blot analysis of SCN7A expression in HeLa (A), mouse heart (B) whole cell lysates.)
Western Blot (WB) (Western blot analysis of SCN7A expression in A549 (A), NIH3T3 (B), H9C2 (C) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC) (Immunofluorescent analysis of SCN7A staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
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