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32 results for "ovarian-specific-serine-threonine-protein-kinase" - showing 1-32


Activity Data (Anti-Müllerian hormone (AMH), also named Müllerian inhibiting substance (MIS) belongs to a tissue-specific TGF-beta superfamily growth factor. It can be expressed by male sertoli cells and postnatal testis, and ovarian granulosa cells of females postpartum. AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. AMH signals through a characteristic receptor consisting of a type I and a type II receptor serine/threonine kinase. Especially the type II receptor is unique and specific receptor for AMH. Besides, Mothers Against Decapentaplegic Homolog 9 (Smad9) has been identified as an interactor of AMH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AMH and recombinant human (Smad9) Briefly, AMH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Smad9-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-AMH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of AMH and Smad9 was shown in Figure 1, and this effect was in a dose dependent manner.)

Anti-Mullerian Hormone (AMH), Active Protein (Cat# AAA2097270)

Full Name
Active Anti-Mullerian Hormone (AMH)
Gene Names
AMH; MIF; MIS
Reactivity
Homo sapiens (Human)
Applications
Cell culture, Activity Assays.
Purity
>95%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Western Blot (WB) (Western blot analysis of Phospho-Aurora Kinase (Thr288) expression in various lysates)

Aurora Kinase, Polyclonal Antibody (Cat# AAA9600783)

Full Name
Phospho-Aurora Kinase (Thr288) Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Aurora Kinase expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Aurora Kinase, Polyclonal Antibody (Cat# AAA9601674)

Full Name
Aurora Kinase Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600773 at 1/100 staining human skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. )

SGK, Polyclonal Antibody (Cat# AAA9600773)

Full Name
Phospho-SGK (Ser422) Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600805 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. )

Chk2, Polyclonal Antibody (Cat# AAA9600805)

Full Name
Phospho-Chk2 (Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of AurA expression in COS7 cells.The lane on the left is treated with the antigen-specific peptide.)

AurA, Polyclonal Antibody (Cat# AAA9601497)

Full Name
AurA Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9607498 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

AURKA, Polyclonal Antibody (Cat# AAA9607498)

Full Name
AURKA Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600627 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

Chk1, Polyclonal Antibody (Cat# AAA9600627)

Full Name
Phospho-Chk1 (Ser280) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot analysis of Jurkat cell and Human fetal kidney tissue using DUSP6 Polyclonal Antibody at dilution of 1:450)

DUSP6, Polyclonal Antibody (Cat# AAA2527027)

Full Name
DUSP6 Polyclonal Antibody
Gene Names
DUSP6; HH19; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC) ((2.5ug/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

PIK3CA/P110alpha, Polyclonal Antibody (Cat# AAA421100)

Full Name
Goat anti-PIK3CA/P110alpha Antibody
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
EIA, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg

Loki/Chk2, Polyclonal Antibody (Cat# AAA422971)

Full Name
Goat anti-Loki/Chk2 (Drosophila melanogaster aa115-128) Antibody
Gene Names
lok; 10895; 38B.4; CG10895; chk; CHK-2; chk2; Chk2; CHK2; chk2/lok; Dmchk2; DmChk2; DmCHk2; DmelCG10895; Dmnk; Dmnk-L; Dmnk-S; Dmnk/DChk2; LOKI/CHK2; mnk; MNK
Reactivity
Expected from sequence similarity: Drosophila
Applications
EIA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$250/0.1 mg

Ovarian-specific serine/threonine-protein kinase Lok (lok), Recombinant Protein (Cat# AAA1001609)

Full Name
Recombinant Drosophila melanogaster Ovarian-specific serine/threonine-protein kinase Lok (lok)
Gene Names
lok; 10895; 38B.4; CG10895; chk; CHK-2; chk2; Chk2; CHK2; chk2/lok; Dmchk2; DmChk2; DmCHk2; DmelCG10895; Dmnk; Dmnk-L; Dmnk-S; Dmnk/DChk2; LOKI/CHK2; mnk; MNK
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$2,175/1 mg (E-Coli) | $2,560/1 mg (Yeast) | $3,735/1 mg (Baculovirus) | $1,920/0.1 mg (Baculovirus) | $2,515/0.1 mg (Mammalian-Cell) | $1,205/0.1 mg (E-Coli) | $1,285/0.1 mg (Yeast) | $6,195/0.5 mg (Mammalian-Cell) | $1,330/0.02 mg (Baculovirus) | $1,600/0.02 mg (Mammalian-Cell) | $1,030/0.02 mg (E-Coli) | $1,125/0.02 mg (Yeast)

Loki / Chk2, Blocking Peptide (Cat# AAA427853)

Full Name
Loki / Chk2 (Drosophila melanogaster aa115-128) Immunizing Peptide
Gene Names
lok; 10895; 38B.4; CG10895; chk; CHK-2; chk2; Chk2; CHK2; chk2/lok; Dmchk2; DmChk2; DmCHk2; DmelCG10895; Dmnk; Dmnk-L; Dmnk-S; Dmnk/DChk2; LOKI/CHK2; mnk; MNK
Reactivity
Drosophila
Pricing
$215/0.1 mg
Western Blot (WB) (Western Blot analysis of RAW264.7 cell using DUSP4 Polyclonal Antibody at dilution of 1:600)

DUSP4, Polyclonal Antibody (Cat# AAA2524092)

Full Name
DUSP4 Polyclonal Antibody
Gene Names
DUSP4; TYP; HVH2; MKP2; MKP-2
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody(MBS179079)BRAF was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BRAF Antibody (MBS179079)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

B Raf, Monoclonal Antibody (Cat# AAA179079)

Full Name
Anti-B Raf Rabbit Monoclonal Antibody
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Aurora A expression in HepG2 cell lysate (MBS179219).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AURKA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AURKA )

Aurora A, Monoclonal Antibody (Cat# AAA179219)

Full Name
Anti-Aurora A Rabbit Monoclonal Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from 293 hydroxyurea, using Phospho-Cyclin E1 (Ser73) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Cyclin E1, Polyclonal Antibody (Cat# AAA9613214)

Full Name
Phospho-Cyclin E1 (Ser73) Antibody
Gene Names
CCNE1; CCNE
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Peptide affinity purification
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using BRSK1 Polyclonal Antibody at dilution of 1:95(×200))

BRSK1, Polyclonal Antibody (Cat# AAA2562386)

Full Name
BRSK1 Polyclonal Antibody
Gene Names
BRSK1; hSAD1
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
Antigen Affinity Purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from various samples, using Phospho-4E-BP1 (Thr37/Thr46) Antibody.Lane 1: B16F10 cells(UV treatment), blocked with antigen-specific peptides,Lane 2: B16F10 cells(UV treatment),Lane 3: P19 cells(serum starvation treatment).Observed bands: 17kDa)

4E-BP1, Polyclonal Antibody (Cat# AAA9612898)

Full Name
Phospho-4E-BP1 (Thr37/Thr46) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DUSP6 antibody.)

DUSP6, Polyclonal Antibody (Cat# AAA6414750)

Full Name
DUSP6 (Dual Specificity Phosphatase 6, HH19, MKP3, PYST1) (Biotin)
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DUSP6 antibody.)

DUSP6, Polyclonal Antibody (Cat# AAA6414752)

Full Name
DUSP6 (Dual Specificity Phosphatase 6, HH19, MKP3, PYST1) (HRP)
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DUSP6 antibody.)

DUSP6, Polyclonal Antibody (Cat# AAA6414749)

Full Name
DUSP6 (Dual Specificity Phosphatase 6, HH19, MKP3, PYST1) (APC)
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using MBS7123664(DUSP6 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200))

DUSP6, Polyclonal Antibody (Cat# AAA7123664)

Full Name
DUSP6 Antibody
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purification
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL

LOK, Polyclonal Antibody (Cat# AAA7181547)

Full Name
Rabbit anti-Drosophila melanogaster (Fruit fly) LOK Polyclonal Antibody
Gene Names
lok; 10895; 38B.4; CG10895; chk; CHK-2; chk2; Chk2; CHK2; chk2/lok; Dmchk2; DmChk2; DmCHk2; DmelCG10895; Dmnk; Dmnk-L; Dmnk-S; Dmnk/DChk2; LOKI/CHK2; mnk; MNK
Reactivity
Drosophila melanogaster (Fruit fly)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DUSP6 antibody.)

DUSP6, Polyclonal Antibody (Cat# AAA6414751)

Full Name
DUSP6 (Dual Specificity Phosphatase 6, HH19, MKP3, PYST1) (FITC)
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Immunohistochemistry (IHC)-Paraffin (The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using MBS7123669(DUSP4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200))

DUSP4, Polyclonal Antibody (Cat# AAA7123669)

Full Name
DUSP4 Antibody
Gene Names
DUSP4; TYP; HVH2; MKP2; MKP-2
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purification
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunohistochemistry (IHC) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

DYRK1A/B, Polyclonal Antibody (Cat# AAA9612576)

Full Name
Phospho-DYRK1A/B (Tyr319/Tyr271) Antibody
Gene Names
DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DUSP6 antibody.)

DUSP6, Polyclonal Antibody (Cat# AAA6414748)

Full Name
DUSP6 (Dual Specificity Phosphatase 6, HH19, MKP3, PYST1) (AP)
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DUSP6 antibody.)

DUSP6, Polyclonal Antibody (Cat# AAA6414758)

Full Name
DUSP6 (Dual Specificity Phosphatase 6, HH19, MKP3, PYST1) (PE)
Gene Names
DUSP6; MKP3; PYST1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
$940/0.1 mL | $4,215/5x0.1 mL
Immunohistochemistry (IHC) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

SRPK2, Polyclonal Antibody (Cat# AAA9612701)

Full Name
Phospho-SRPK2 (Ser494/Ser497) Antibody
Gene Names
SRPK2; SFRSK2
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of VPRBP/DCAF1 using anti-VPRBP/DCAF1 antibody (MBS1753850).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: rat testis tissue lysatesLane 5: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-VPRBP/DCAF1 antigen affinity purified polyclonal antibody (Catalog # MBS1753850) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for VPRBP/DCAF1 at approximately 180KD. The expected band size for VPRBP/DCAF1 is at 180KD. )

VPRBP/DCAF1, Polyclonal Antibody (Cat# AAA1753850)

Full Name
Anti-VPRBP/DCAF1 Antibody
Gene Names
DCAF1; RIP; VPRBP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of ALK-1/ACVRL1 using anti-ALK-1/ACVRL1 antibody (MBS1753435).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: rat brain tissue lysatesLane 4: rat heart tissue lysatesLane 5: mouse brain tissue lysatesLane 6: mouse kidney tissue lysatesLane 7: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ALK-1/ACVRL1 antigen affinity purified polyclonal antibody (Catalog # MBS1753435) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ALK-1/ACVRL1 at approximately 55KD. The expected band size for ALK-1/ACVRL1 is at 55KD. )

ALK-1/ACVRL1, Polyclonal Antibody (Cat# AAA1753435)

Full Name
Anti-ALK-1/ACVRL1 Antibody
Gene Names
ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
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