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13 results for " dnmts" - showing 1-13
Western Blot (WB)
(Figure 1. Western blot analysis of Dnmt1 using anti-Dnmt1 antibody (MBS1750342).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-937 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200KD. The expected band size for Dnmt1 is at 183KD. )
Dnmt1, Polyclonal Antibody (Cat# AAA1750342)
Full Name
Anti-Dnmt1 Picoband antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Dnmt1 expression in (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate (MBS179160))
Dnmt1, Monoclonal Antibody (Cat# AAA179160)
Full Name
Anti-Dnmt1 Rabbit Monoclonal Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MGMT expression in HeLa cell lysate (MBS179520).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGMT monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MGMT )
MGMT, Monoclonal Antibody (Cat# AAA179520)
Full Name
Anti-MGMT Rabbit Monoclonal Antibody
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Dnmt3b expression in (1) A549 cell lysate; (2) A431 cell lysate (MBS179261).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNMT3B )
Dnmt3b, Monoclonal Antibody (Cat# AAA179261)
Full Name
Anti-Dnmt3b Rabbit Monoclonal Antibody Rabbit Monoclonal Antibody
Gene Names
DNMT3B; ICF; ICF1; M.HsaIIIB
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Dnmt3a expression in HeLa cell lysate (MBS179134).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNMT3A )
Dnmt3a, Monoclonal Antibody (Cat# AAA179134)
Full Name
Anti-Dnmt3a Rabbit Monoclonal Antibody
Gene Names
DNMT3A; TBRS; DNMT3A2; M.HsaIIIA
Reactivity
Human, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Anti-MGMT rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:22 kDa)
MGMT, Polyclonal Antibody (Cat# AAA8119359)
Full Name
Anti-MGMT Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
WB, IP
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Immunohistochemistry (IHC)
(Immunochemical staining of human DNMT3A in human hepatoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
DNMT3A, Polyclonal Antibody (Cat# AAA8100686)
Full Name
Anti-DNMT3A Antibody, Rabbit Polyclonal
Gene Names
DNMT3A; DNMT3A2; M.HsaIIIA
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
IHC-P, ICC, IF
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Western Blot (WB)
(Anti-DNMT1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:183 kDa)
DNMT1, Polyclonal Antibody (Cat# AAA8108407)
Full Name
Anti-DNMT1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
WB, IHC-P, ICC, IF
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
SDS-Page
DNMT2, Recombinant Protein (Cat# AAA8120748)
Full Name
DNMT2 Protein, Human, Recombinant (GST Tag)
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Purity
>94% as determined by SDS-PAGE
Pricing
$450/0.1 mg | $1,885/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of TRDMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TRDMT1 polyclonal antibody (dilution ratio 1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.)
DNMT2, Polyclonal Antibody (Cat# AAA8101385)
Full Name
Anti-DNMT2 Antibody, Rabbit Polyclonal
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Reactivity
Human
Applications
ICC, IF
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescence staining of DNMT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human DNMT1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)
DNMT1, Polyclonal Antibody (Cat# AAA8100682)
Full Name
Anti-DNMT1 Antibody, Rabbit Polyclonal
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human
Applications
IHC-P, ICC, IF
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Immunohistochemistry (IHC)
(Immunochemical staining of human DNMT3A in human hepatoma with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)
DNMT3A, Polyclonal Antibody (Cat# AAA8100685)
Full Name
Anti-DNMT3A Antibody, Rabbit Polyclonal
Gene Names
DNMT3A; DNMT3A2; M.HsaIIIA
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
IHC-P
Purity
Protein A & Antigen Affinity
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
DNMT2, Cell Lysate (Cat# AAA8114484)
Full Name
Human DNMT2 Baculovirus-Insect cells Overexpression Lysate
Gene Names
TRDMT1; DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
Applications
WB
Pricing
$280/0.3 mg | $1,205/5x0.3 mg
