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329 results for " Stem Cell Signaling Pathways" - showing 1-50


KIT, Monoclonal Antibody (Cat# AAA9200373)

Full Name
KIT Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
WB, EIA, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot detection of BRG1 in Hela NE, F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.)

BRG1, Monoclonal Antibody (Cat# AAA475232)

Full Name
Anti-BRG1 Mouse mAb
Gene Names
SMARCA4; BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
Reactivity
Human, Mouse
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunofluorescence (IF) (Confocal immunofluorescence image of HepG2 cells stained with GPX4/MCSP Mouse Monoclonal Antibody (LHM 2) followed by Goat anti-Mouse CF488 (green). Reddot is used to label the nuclei red.)

GPX4/MCSP, Monoclonal Antibody (Cat# AAA4380953)

Full Name
GPX4/MCSP
Gene Names
GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (MBS9200563 staining CSF1R in human skin sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at roomtemperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylatedgoat polyvalent antibody was used as the secondary antibody.)

CSF1R, Monoclonal Antibody (Cat# AAA9200563)

Full Name
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
WB, EIA, FC, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot detection of Wnt3a in MCF-7, ZR75-1, MDA-MB-468 and T47D cell lysates using Wnt3a mouse mAb (1:1000 diluted).Predicted band size: 42KDa.Observed band size:42KDa.)

Wnt3a, Monoclonal Antibody (Cat# AAA475532)

Full Name
Anti-Wnt3a Mouse mAb
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Anti-c-KIT Antibody (N-term) at 1:1000dilution+ Mouse cerebellum tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

c-KIT, Polyclonal Antibody (Cat# AAA9204642)

Full Name
c-KIT Antibody (N-term)
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human, Monkey
Applications
WB, EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

FMR1, Polyclonal Antibody (Cat# AAA9207834)

Full Name
FMR1 Antibody (N-term)
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, mouse
Applications
IHC-P, WB, FC,E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Figure 1. Western blot analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (MBS1750890).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: 293T whole Cell lysates,Lane 5: 293T whole Cell lysates,Lane 6: HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Musashi 1/Msi1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Musashi 1/Msi1 at approximately 39KD. The expected band size for Musashi 1/Msi1 is at 39KD. )

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA1750890)

Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)

Serum Albumin, Monoclonal Antibody (Cat# AAA475187)

Full Name
Anti-Human Serum Albumin Mouse mAb
Gene Names
ALB; HSA; PRO0883; PRO0903; PRO1341
Reactivity
Human
Applications
WB, EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of Frizzled 4 using anti-Frizzled 4 antibody (MBS1750751).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human MCF-7 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Frizzled 4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Frizzled 4 at approximately 65KD. The expected band size for Frizzled 4 is at 60KD. )

Frizzled 4, Polyclonal Antibody (Cat# AAA1750751)

Full Name
Anti-Frizzled 4 Picoband antibody
Gene Names
FZD4; Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of FZD3 using anti-FZD3 antibody (MBS1750879).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SK-OV-3 cell lysates,Lane 2: human Jurkat cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FZD3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FZD3 at approximately 76KD. The expected band size for FZD3 is at 76KD. )

FZD3/Frizzled 3, Polyclonal Antibody (Cat# AAA1750879)

Full Name
Anti-FZD3/Frizzled 3 antibody
Gene Names
FZD3; Fz-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of 14-3-3 expression in Hela lysate (MBS179850).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB )

14-3-3, Monoclonal Antibody (Cat# AAA179850)

Full Name
Anti-14-3-3 Rabbit Monoclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) PC-12 cell lysate (MBS179851).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB )

14-3-3, Monoclonal Antibody (Cat# AAA179851)

Full Name
Anti-14-3-3 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Raji cell lysate.)

STAT3, Monoclonal Antibody (Cat# AAA179045)

Full Name
Anti-STAT3 Rabbit Monoclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
FC, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa. Other Band(s): due to post translational modifications.)

c-Myc, Polyclonal Antibody (Cat# AAA8000514)

Full Name
c-Myc Antibody: PerCP
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000960)

Full Name
CBP Antibody: Biotin
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB) (Western blot analysis of 14-3-3 alpha + beta expression in Hela lysate (MBS179852).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAB )

14-3-3 alpha + beta, Monoclonal Antibody (Cat# AAA179852)

Full Name
Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 sigma expression in A431 cell lysate (MBS179566).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFN )

14-3-3 sigma, Monoclonal Antibody (Cat# AAA179566)

Full Name
Anti-14-3-3 sigma Rabbit Monoclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Bovine Serum Albumin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate (MBS179606).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALB )

Serum Albumin, Monoclonal Antibody (Cat# AAA179606)

Full Name
Anti-Bovine Serum Albumin Rabbit Monoclonal Antibody
Gene Names
ALB; HSA; PRO0883; PRO0903; PRO1341
Reactivity
Reactive Species: Cow ,Human, Mouse, Rat (Predicted Species: Hamster)
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of 14-3-3 epsilon expression in 293T cell lysate (MBS179716).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAE monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAE )

14-3-3 epsilon, Monoclonal Antibody (Cat# AAA179716)

Full Name
Anti-14-3-3 epsilon Rabbit Monoclonal Antibody
Gene Names
YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF (MBS1750178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT3 )

STAT3, Monoclonal Antibody (Cat# AAA1750178)

Full Name
Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

THBS1/TSP1, ELISA Kit (Cat# AAA1751079)

Full Name
Monkey primate THBS1/TSP1 PicoKine ELISA Kit
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Reactivity
Monkey
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000962)

Full Name
CBP Antibody: HRP
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of Dishevelled 2 using anti- Dishevelled 2 antibody (MBS1750766).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Dishevelled 2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dishevelled 2 at approximately 79KD. The expected band size for Dishevelled 2 is at 79KD. )

Dishevelled 2, Polyclonal Antibody (Cat# AAA1750766)

Full Name
Anti-Dishevelled 2 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of STAT3 expression in Raji cell lysate (MBS179046).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT3 )

STAT3, Monoclonal Antibody (Cat# AAA179046)

Full Name
Anti-STAT3 Rabbit Monoclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of ADAM17 expression in Hela cell lysate (MBS179386).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAM17 )

ADAM17/Tace, Monoclonal Antibody (Cat# AAA179386)

Full Name
Anti-ADAM17/Tace Rabbit Monoclonal Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000951)

Full Name
CBP Antibody: ATTO 488
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa.)

c-Myc, Polyclonal Antibody (Cat# AAA8000493)

Full Name
c-Myc Antibody: FITC
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody (SPC-737) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band(s): ~35 kDa isoform 2.)

Wnt5a, Polyclonal Antibody (Cat# AAA8000710)

Full Name
Wnt5a Antibody: HRP
Gene Names
WNT5A; hWNT5A
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-725) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa.)

c-Myc, Polyclonal Antibody (Cat# AAA8000483)

Full Name
c-Myc Antibody: ATTO 488
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate (MBS1750006).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAG monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YWHAG )

14-3-3 gamma, Monoclonal Antibody (Cat# AAA1750006)

Full Name
Anti-14-3-3 gamma Rabbit Monoclonal Antibody
Gene Names
YWHAG; EIEE56; PPP1R170; 14-3-3GAMMA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000953)

Full Name
CBP Antibody: ATTO 594
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000964)

Full Name
CBP Antibody: PerCP
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB) (Western blot detection of Wnt5b in CHO-K1 cell lysate (B) and CHO-K1 transfected by Wnt5b (A) cell lysate using Wnt5b mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size:45KDa.)

Wnt5b, Monoclonal Antibody (Cat# AAA475540)

Full Name
Anti-Wnt5b Mouse mAb
Reactivity
Transfected
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000965)

Full Name
CBP Antibody: RPE
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000950)

Full Name
CBP Antibody: ATTO 390
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000958)

Full Name
CBP Antibody: Alkaline Phosphatase
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000949)

Full Name
CBP Antibody
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of ADAM17 using anti-ADAM17 antibody (MBS1750443).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM17 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAM17 at approximately 78KD. The expected band size for ADAM17 is at 97KD. )

ADAM17/Tace, Polyclonal Antibody (Cat# AAA1750443)

Full Name
Anti-ADAM17/Tace Picoband Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.)

STAT3, Monoclonal Antibody (Cat# AAA1750179)

Full Name
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-751). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-751) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000961)

Full Name
CBP Antibody: FITC
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Testing Data (Pomalidomide is tumor necrosis factor-alpha inhibitor and a new immunomodulator with an IC50 of 1 uM. It is a derivative of thalidomide and is 10,000 times more potent than thalidomide in terms of inhibition of tumor necrosis factor-alpha. Additionally, it helps to restrict tumors' necessary blood vessel growth. Compared to thalidomide, pomalidomide has demonstrated enhanced immunological effects in lab testing, including an approximately 500-2,000 times greater potency at stimulating the proliferation of T-cells. Like thalidomide, pomalidomide appears to function through multiple pathways to inhibit myeloma cells' growth and survival. [1][2])

Pomalidomide, Inhibitor (Cat# AAA8506593)

Full Name
Pomalidomide
Purity
>99%
Pricing
$440/10 mg | $1,940/5x10 mg
Western Blot (WB) (Western blot analysis of STAT3 expression in A431 cell lysate (MBS179047).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT3 )

STAT3, Monoclonal Antibody (Cat# AAA179047)

Full Name
Anti-STAT3 Rabbit Monoclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Phospho-Smad3 (S423/S425) expression in PC-12 cell lysate treated with 1% BSA.)

Smad3, Monoclonal Antibody (Cat# AAA1750200)

Full Name
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000976)

Full Name
CBP Antibody: Alkaline Phosphatase
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney, using Smad1 Antibody(MBS179428)SMAD1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD1 Antibody (MBS179428)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Smad1, Monoclonal Antibody (Cat# AAA179428)

Full Name
Anti-Smad1 Rabbit Monoclonal Antibody
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human, Mouse
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000979)

Full Name
CBP Antibody: FITC
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB) (Western blot analysis of Gli1 expression in A549 cell lysate (MBS179353).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI1 )

Gli1, Monoclonal Antibody (Cat# AAA179353)

Full Name
Anti-Gli1 Rabbit Monoclonal Antibody
Gene Names
GLI1; GLI
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)

CBP, Polyclonal Antibody (Cat# AAA8000982)

Full Name
CBP Antibody: PerCP
Gene Names
CREBBP; CBP; RSTS; KAT3A; RSTS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB) (Western blot analysis of HDAC3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179471).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC3 )

HDAC3, Monoclonal Antibody (Cat# AAA179471)

Full Name
Anti-HDAC3 Rabbit Monoclonal Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
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