Typical Testing Data/Standard Curve (for reference only)
Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit | RPE65 elisa kit
Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit
Reactivity
Mouse
Synonyms
Retinoid isomerohydrolase (RPE65); Mouse Retinoid isomerohydrolase (RPE65) ELISA Kit; RPE65 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of RPE65. No significant cross-reactivity or interference between RPE65 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPE65 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RPE65 elisa kit
Intended Uses: This RPE65 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RPE65. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: RPE65 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RPE65 antibody and an RPE65-HRP conjugate. The assay sample and buffer are incubated together with RPE65-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPE65 concentration since RPE65 from samples and RPE65-HRP conjugate compete for the anti-RPE65 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPE65 from the sample, fewer sites are left to bind RPE65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPE65 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: RPE65 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RPE65 antibody and an RPE65-HRP conjugate. The assay sample and buffer are incubated together with RPE65-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPE65 concentration since RPE65 from samples and RPE65-HRP conjugate compete for the anti-RPE65 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPE65 from the sample, fewer sites are left to bind RPE65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPE65 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,930 Da
NCBI Official Full Name
retinoid isomerohydrolase
NCBI Official Synonym Full Names
retinal pigment epithelium-specific protein 65kDa
NCBI Official Symbol
RPE65
NCBI Protein Information
retinoid isomerohydrolase; retinol isomerase; all-trans-retinyl-palmitate hydrolase; retinal pigment epithelium-specific 65 kDa protein
UniProt Protein Name
Retinoid isomerohydrolase
UniProt Gene Name
RPE65
UniProt Entry Name
RPE65_CHICK
Uniprot Description
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
Research Articles on RPE65
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Product Notes
The Mouse RPE65 rpe65 (Catalog #AAA7214137) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214137 ELISA Kit recognizes Mouse RPE65. It is sometimes possible for the material contained within the vial of "Retinoid isomerohydrolase (RPE65), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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