$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
Western Blot (WB) ((0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) (Western blot analysis of Hsp47 expression in HeLa cell lysate (MBS179932).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINH1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SERPINH1 )
Western Blot (WB) (Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)
Western Blot (WB) (Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)
Western Blot (WB) (Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)
Western Blot (WB) (Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)
Western Blot (WB) (Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)
Western Blot (WB) (Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)
Western Blot (WB) (Western blot detection of PSMB10 in C6 cell lysates using PSMB10 Rabbit mAb(1:500 diluted). Predicted band size:29kDa. Observed band size:29kDa.)
SDS-Page (SDS-Page)
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