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5 results for " Myogenesis Metabolism" - showing 1-5
Sequence
Myostatin (MSTN), Active Protein (Cat# AAA2105145)
Full Name
Active Myostatin (MSTN)
Gene Names
Mstn; Cmpt; Gdf8
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 95%
Pricing
$170/0.01 mg | $320/0.05 mg | $500/0.1 mg | $615/0.2 mg | $1,180/0.5 mg | $1,755/1 mg | $4,300/5 mg
Activity Data
(Figure. The binding activity of MSTN with FSTL3.Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by myocytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat MSTN and recombinant rat FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Myostatin (MSTN), Active Protein (Cat# AAA2105146)
Full Name
Active Myostatin (MSTN)
Gene Names
Mstn; Gdf8
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $500/0.05 mg | $770/0.1 mg | $955/0.2 mg | $1,860/0.5 mg | $2,755/1 mg | $6,740/5 mg
Testing Data
(Standard Curve for H-FABP: Capture Antibody Mouse mAb (5C4-F5) to H-FABP at 4mug/ml and Detector Antibody Mouse mAb (3E8-B10?7B10-G11?4H1-B8)to H-FABP at 0.08 mug/ml.)
H-FABP, Monoclonal Antibody (Cat# AAA475191)
Full Name
Anti-H-FABP Mouse mAb
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Applications
sELISA Capture
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Standard Curve for H-FABP: Capture Antibody Mouse mAb (5C4-F5) to H-FABP at 4mug/ml and Detector Antibody Mouse mAb (3E8-B10?7B10-G11?4H1-B8)to H-FABP at 0.08 mug/ml.)
H-FABP, Monoclonal Antibody (Cat# AAA475219)
Full Name
Anti-H-FABP Mouse mAb
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Applications
sELISA Detector
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (MBS1750703).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD. )
Cardiac FABP, Polyclonal Antibody (Cat# AAA1750703)
Full Name
Anti-Cardiac FABP Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
