Rabbit anti-Human, Mouse GDF10 Polyclonal Antibody | anti-GDF10 antibody

GDF10, ID (Bone Morphogenetic Protein 3B, BMP-3B, Growth/Differentiation Factor 10, GDF-10, Bone-inducing Protein, BIP, BMP3B) (Azide free) (HRP)

Cat. Num. AAA6465543

• Gene Names: GDF10; BIP; BMP3B; BMP-3b
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: GDF10; Polyclonal Antibody; ID (Bone Morphogenetic Protein 3B; BMP-3B; Growth/Differentiation Factor 10; GDF-10; Bone-inducing Protein; BIP; BMP3B) (Azide free) (HRP); anti-GDF10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GDF10. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GDF10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human GDF10 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GDF10 Antibody (Center) in NIH-3T3 cell line lysates (35ug/lane). GDF10 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using GDF10 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GDF10 antibody

Antibodies; Growth Factors; Cytokines

References

1. Ducy, P., et al., Kidney Int. 57(6):2207-2214 (2000). 2. Hino, J., et al., Biochem. Biophys. Res. Commun. 223(2):304-310 (1996). 3. Cunningham, N.S., et al., Growth Factors 12(2):99-109 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4826740
• NCBI GeneID: 2662
• NCBI Accession #: NP_004953
• NCBI GenBank Nucleotide #: NM_004962.4
• UniProt Accession #: P55107; Q5VSQ8; Q9UCX6
• Molecular Weight: 12.5 kDa (111aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: growth/differentiation factor 10 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 10
• NCBI Official Symbol: GDF10
• NCBI Official Synonym Symbols: BIP; BMP3B; BMP-3b
• NCBI Protein Information: growth/differentiation factor 10
• UniProt Protein Name: Bone morphogenetic protein 3B
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF10
• UniProt Synonym Gene Names: BMP3B; BMP-3B; BIP; GDF-10
• UniProt Entry Name: BMP3B_HUMAN

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. Additionally, this protein may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer. [provided by RefSeq, Jul 2016]

Uniprot Description

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; fat cell differentiation; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; cell development; skeletal development; growth