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8 results for " Metaboli" - showing 1-8
Western Blot (WB)
(Western blot detection of Fatty Acid Synthase in Hela, C6, 3T3, CHO-K1 and MCF7 cell lysates using Fatty Acid Synthase mouse mAb (dilution 1:500).Predicted band size:273kDa.Observed band size:273kDa.)
Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA475287)
Full Name
Anti-Fatty Acid Synthase Mouse mAb
Gene Names
FASN; FAS; OA-519; SDR27X1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IP, IF
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of FASN expression in A549 cell lysate (MBS179494).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FASN )
FASN/Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA179494)
Full Name
Anti-FASN/Fatty Acid Synthase Rabbit Monoclonal Antibody
Gene Names
FASN; FAS; OA-519; SDR27X1
Reactivity
Human, Mouse, Rat
Applications
FC, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using ENO1 Antibody(MBS179609)ENO1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ENO1 Antibody (MBS179609)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
ENO1/Alpha Enolase, Monoclonal Antibody (Cat# AAA179609)
Full Name
Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ERR alpha expression in HeLa cell lysate (MBS179952).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ESRRA )
ERR alpha, Monoclonal Antibody (Cat# AAA179952)
Full Name
Anti-ERR alpha Rabbit Monoclonal Antibody
Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of BNIP3L expression in K562 cell lysate (MBS179936).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNIP3L monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BNIP3L )
BNIP3L/Nix, Monoclonal Antibody (Cat# AAA179936)
Full Name
Anti-BNIP3L/Nix Rabbit Monoclonal Antibody
Gene Names
BNIP3L; NIX; BNIP3a
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-kB p65 Antibody(MBS179238)RELA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RELA Antibody (MBS179238)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
NF-Kappa B p65, Monoclonal Antibody (Cat# AAA179238)
Full Name
Anti-NF-Kappa B p65 Rabbit Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Bax in (1) HeLa cell lysate;(2) RAW2647 Whole Cell Lysate (MBS179170).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAX monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAX )
Bax, Monoclonal Antibody (Cat# AAA179170)
Full Name
Anti-Bax Rabbit Monoclonal Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-NF-Kappa B p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha (MBS1750229).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RELA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RELA )
NF-Kappa B p65, Monoclonal Antibody (Cat# AAA1750229)
Full Name
Anti-Phospho-NF-Kappa B p65 (S529) Rabbit Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
